GFAP antibody

Catalog No. ABIN967502

Product Details anti-GFAP Antibody

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Pig, Cow, Chicken, Rabbit, Guinea Pig, Sheep, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: LEP-02

Isotype: IgG2b

Application Details

Application Notes: Applications include indirect immunofluorescence of tissue-cultured cells, immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (10-15 µg/ml), and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control. Antibodies-online offers these GFAP specific antibodies separately: clone 4A11 (ABIN967499), clone 1B4 (ABIN967500) and clone 2E1 (ABIN967501).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-GFAP antibody (ABIN967502)

McLendon, Bigner: "Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).

McLendon, Burger, Pegram, Eng, Bigner: "The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues." in: Journal of neuropathology and experimental neurology, Vol. 45, Issue 6, pp. 692-703, (1986) (PubMed).

Pegram, Eng, Wikstrand, McComb, Lee, Bigner: "Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species." in: Neurochemical pathology, Vol. 3, Issue 2, pp. 119-38, (1985) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP (GFAP Products)

Synonyms: GFAP antibody, AI836096 antibody, cb345 antibody, etID36982.3 antibody, gfapl antibody, wu:fb34h11 antibody, wu:fk42c12 antibody, xx:af506734 antibody, zgc:110485 antibody, glial fibrillary acidic protein antibody, GFAP antibody, LOC100136168 antibody, gfap antibody, Gfap antibody

Background: GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. We offer a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Bovine spinal cord homogenate was used as immunogen for these clones. Clones 4A11, 1B4, and 2E1 have broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken. The cocktail preparation was made by combining all three antibodies in equal concentrations. The antibodies are routinely tested by immunohistochemical staining of formalin-fixed, paraffin-embedded brain tissue samples, which were subjected to pretreatment with citrate or tissue unmasking fluid (see figure).

Molecular Weight: 50 kDa