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Spleen tyrosine Kinase (SYK) (pTyr348) antibody

Details for Product No. ABIN967580, Supplier: Log in to see
Antigen
  • p72-Syk
  • p72syk
  • Sykb
Alternatives
anti-Human Spleen tyrosine Kinase antibody for Immunocytochemistry
Epitope
pTyr348
49
33
30
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Reactivity
Human, Mouse (Murine), Rat (Rattus)
344
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Host
Mouse
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Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
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Application
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Intracellular Staining (ICS), Western Blotting (WB)
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Supplier
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Brand BD Pharmingen™
Immunogen Phosphorylated Human Syk Peptide
Clone I120-722
Isotype IgG1, kappa
Cross-Reactivity (Details) Predicted: Mouse, Rat
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Syk (SYK Antibody Abstract)
Background Syk is a non-receptor protein-tyrosine kinase that is closely related to ZAP70 and plays crucial roles in the development and receptor-mediated signaling of most leukocytes and in vascular integrity. Syk is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. Mature (CD4 or CD8 single-positive) thymocytes and peripheral alphabeta TCR-bearing T lymphocytes have very low or undetectable levels of Syk. Syk contributes to the signal transduction process by binding to ITAMs (Immunoreceptor Tyrosine-based Activation Motifs) of immune receptors, including Igalpha and Igbeta (CD79a and b), TCRzeta, CD3epsilon, and FcRgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in the interdomain, between the SH2 and Kinase domains of Syk, to undergo auto-phosphorylation and phosphorylation by Lyn. The tyrosine 348 phosphorylation site (pY348) in human Syk is orthologous to tyrosine 342 in mouse and rat Syk and tyrosine 315 in human ZAP70. This phosphorylated site can act as a binding site for other signaling molecules, such as PLCgamma, Vav, and Fgr. The I120-772 antibody is specific for human Syk (pY348) and does not cross-react with phosphorylated Zap70. The orthologous phosphorylation site in mouse and rat Syk is Y342.
Molecular Weight 72 kDa
Research Area Immunology, Innate Immunity, Tyrosine Kinases, Signaling
Pathways Fc-epsilon Receptor Signaling Pathway
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
 image for anti-Spleen tyrosine Kinase (SYK) (pTyr348) antibody (ABIN967580) Lysate from control (First Panel) and pervanadate-treated (Second Panel) Ramos cells ...
 image for anti-Spleen tyrosine Kinase (SYK) (pTyr348) antibody (ABIN967580) Syk (pY348) staining on tonsil. Fresh human tonsil, stimulated with a 5 mM Pervanadat...
Product cited in: Abtahian, Guerriero, Sebzda, Lu, Zhou, Mocsai, Myers, Huang, Jackson, Ferrari, Tybulewicz, Lowell, Lepore, Koretzky, Kahn: "Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk." in: Science (New York, N.Y.), Vol. 299, Issue 5604, pp. 247-51, 2003

Hong, Yankee, Harrison, Geahlen: "Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. A mechanism for negative signaling by the Lyn tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 31703-14, 2002

Zhang, Berenstein, Siraganian: "Phosphorylation of Tyr342 in the linker region of Syk is critical for Fc epsilon RI signaling in mast cells." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8144-54, 2002

Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, 2001

Vines, Potter, Xu, Geahlen, Costello, Tybulewicz, Lowell, Chang, Gresham, Willman: "Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr." in: Immunity, Vol. 15, Issue 4, pp. 507-19, 2001

Coopman, Do, Barth, Bowden, Hayes, Basyuk, Blancato, Vezza, McLeskey, Mangeat, Mueller: "The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells." in: Nature, Vol. 406, Issue 6797, pp. 742-7, 2000

Turner, Schweighoffer, Colucci, Di Santo, Tybulewicz: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000