SYK antibody (pTyr348)

Catalog No. ABIN967580

Product Details anti-SYK Antibody

Target: SYK (Spleen tyrosine Kinase (SYK))

Binding Specificity: pTyr348

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SYK antibody is un-conjugated

Application: Western Blotting (WB), Intracellular Staining (ICS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Brand: BD Pharmingen™

Cross-Reactivity (Details): Predicted: Mouse, Rat

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human Syk Peptide

Clone: I120-722

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-SYK antibody (ABIN967580)

Abtahian, Guerriero, Sebzda, Lu, Zhou, Mocsai, Myers, Huang, Jackson, Ferrari, Tybulewicz, Lowell, Lepore, Koretzky, Kahn: "Regulation of blood and lymphatic vascular separation by signaling proteins SLP-76 and Syk." in: Science (New York, N.Y.), Vol. 299, Issue 5604, pp. 247-51, (2003) (PubMed).

Hong, Yankee, Harrison, Geahlen: "Regulation of signaling in B cells through the phosphorylation of Syk on linker region tyrosines. A mechanism for negative signaling by the Lyn tyrosine kinase." in: The Journal of biological chemistry, Vol. 277, Issue 35, pp. 31703-14, (2002) (PubMed).

Zhang, Berenstein, Siraganian: "Phosphorylation of Tyr342 in the linker region of Syk is critical for Fc epsilon RI signaling in mast cells." in: Molecular and cellular biology, Vol. 22, Issue 23, pp. 8144-54, (2002) (PubMed).

Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, (2001) (PubMed).

Vines, Potter, Xu, Geahlen, Costello, Tybulewicz, Lowell, Chang, Gresham, Willman: "Inhibition of beta 2 integrin receptor and Syk kinase signaling in monocytes by the Src family kinase Fgr." in: Immunity, Vol. 15, Issue 4, pp. 507-19, (2001) (PubMed).

Coopman, Do, Barth, Bowden, Hayes, Basyuk, Blancato, Vezza, McLeskey, Mangeat, Mueller: "The Syk tyrosine kinase suppresses malignant growth of human breast cancer cells." in: Nature, Vol. 406, Issue 6797, pp. 742-7, (2000) (PubMed).

Turner, Schweighoffer, Colucci, Di Santo, Tybulewicz: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, (2000) (PubMed).

Target Details for SYK

Target: SYK (Spleen tyrosine Kinase (SYK))

Alternative Name: Syk (SYK Products)

Synonyms: p72-Syk antibody, Sykb antibody, p72syk antibody, spleen associated tyrosine kinase antibody, spleen tyrosine kinase antibody, syk antibody, SYK antibody, Syk antibody

Background: Syk is a non-receptor protein-tyrosine kinase that is closely related to ZAP70 and plays crucial roles in the development and receptor-mediated signaling of most leukocytes and in vascular integrity. Syk is expressed in hematopoietic cells, including B lymphocytes, immature (CD4, CD8 double-negative and double-positive) thymocytes, and myeloid cells, epithelial cell lines, and normal breast tissue. Mature (CD4 or CD8 single-positive) thymocytes and peripheral alphabeta TCR-bearing T lymphocytes have very low or undetectable levels of Syk. Syk contributes to the signal transduction process by binding to ITAMs (Immunoreceptor Tyrosine-based Activation Motifs) of immune receptors, including Igalpha and Igbeta (CD79a and b), TCRzeta, CD3epsilon, and FcRgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in the interdomain, between the SH2 and Kinase domains of Syk, to undergo auto-phosphorylation and phosphorylation by Lyn. The tyrosine 348 phosphorylation site (pY348) in human Syk is orthologous to tyrosine 342 in mouse and rat Syk and tyrosine 315 in human ZAP70. This phosphorylated site can act as a binding site for other signaling molecules, such as PLCgamma, Vav, and Fgr. The I120-772 antibody is specific for human Syk (pY348) and does not cross-react with phosphorylated Zap70. The orthologous phosphorylation site in mouse and rat Syk is Y342.

Molecular Weight: 72 kDa

Pathways: Fc-epsilon Receptor Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Thromboxane A2 Receptor Signaling, BCR Signaling