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B-Cell Linker (BLNK) (pTyr84) antibody

Details for Product No. ABIN967596, Supplier: Log in to see
Antigen
  • BLNK
  • blnk
  • MGC147045
  • BASH
  • Bca
  • Ly-57
  • Ly57
  • Lyw-57
  • SLP-65
  • AGM4
  • BLNK-S
  • LY57
  • SLP65
  • bca
Epitope
pTyr84
24
23
18
16
13
13
13
11
10
8
4
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
177
92
59
4
4
4
3
1
1
Host
Mouse
148
24
16
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
7
7
7
6
6
5
4
4
4
4
4
4
4
3
3
Application
Western Blotting (WB)
128
65
44
38
19
8
8
7
6
4
3
1
1
1
Supplier
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Brand BD Pharmingen™
Immunogen Phosphorylated Human BLNK Peptide
Clone J117-1278
Isotype IgG2b, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Please refer to us for technical protocols.
2. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name BLNK (BLNK Antibody Abstract)
Background B cell activation is initiated by crosslinking the B cell receptor, which leads to activation of non-receptor protein tyrosine kinases (PTK), including Btk, Syk, and three Src kinases, Fyn, Lyn, and Blk. Activated PTKs then phosphorylate multiple cellular proteins involved in B lymphocyte signaling. Syk is responsible for the tyrosine phosphorylation of B cell linker protein (BLNK), a member of the SLP-76 family of adapter proteins. Phosphorylation of human BLNK at tyrosines 84, 178, and 189 (Y84, Y178, and Y189) creates docking sites for PLCg2, leading to the activation of downstream signaling pathways. The J117-1278 monoclonal antibody recognizes the phosphorylated Y84 of BLNK.
Molecular Weight 68 kDa
Research Area Signaling, Adaptive Immunity
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Western Blotting (WB) image for anti-B-Cell Linker (BLNK) (pTyr84) antibody (ABIN967596) Western blot analysis of BLNK (pY84) in human Burkitt’s lymphoma. Lysates from contro...
Product cited in: Pighi, Gu, Dalai et al.: "Phospho-proteomic analysis of mantle cell lymphoma cells suggests a pro-survival role of B-cell receptor signaling." in: Cellular oncology (Dordrecht), Vol. 34, Issue 2, pp. 141-53, 2011 (PubMed).

Espeli, Mancini, Breton et al.: "Impaired B-cell development at the pre-BII-cell stage in galectin-1-deficient mice due to inefficient pre-BII/stromal cell interactions." in: Blood, Vol. 113, Issue 23, pp. 5878-86, 2009 (PubMed).

Wu, Koretzky: "The SLP-76 family of adapter proteins." in: Seminars in immunology, Vol. 16, Issue 6, pp. 379-93, 2004 (PubMed).

Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003 (PubMed).

Chiu, Dalton, Ishiai et al.: "BLNK: molecular scaffolding through 'cis'-mediated organization of signaling proteins." in: The EMBO journal, Vol. 21, Issue 23, pp. 6461-72, 2002 (PubMed).