IFN-gamma Receptor (Chain beta) antibody

Details for Product No. ABIN967655, Supplier: Log in to see
Antigen
Epitope
Chain beta
Reactivity
Mouse (Murine)
Host
Armenian Hamster
Clonality (Clone)
Monoclonal ()
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
Options
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Brand BD Pharmingen™
Immunogen Mouse IFN-gammaRbeta chain protein
Clone MOB-47
Isotype IgG
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Although hamster immunoglobulin isotypes have not been well defined, BD Biosciences Pharmingen has grouped Armenian and Syrian hamster IgG monoclonal antibodies according to their reactivity with a panel of mouse anti-hamster IgG mAbs.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Background The MOB-47 antibody recognizes the 60-65 kD beta chain subunit of the mouse interferon-gamma receptor (IFN-gammaRbeta). The IFN-gamma receptor beta chain is expressed by a variety of normal mouse cell types and cell lines that are responsive to IFN-gamma including T and B lymphocytes, NK cells, monocytes, macrophages, granulocytes and fibroblasts. The IFN-gammaRbeta receptor epitope recognized by the MOB-47 antibody is blocked by bound IFN-gamma. The immunogen used to generate this hybridoma was the purified soluble extracellular domain of the mouse IFN-gammaRbeta chain protein.
Application Notes Immunofluorescent Staining and Flow Cytometric Analysis: The purified form of MOB-47 (ABIN967655) can be used for the immunofluorescent staining (Less or equal than 1 µg antibody/10e6 cells) and flow cytometric analysis of normal mouse cells or cell lines to measure their expressed levels of IFN-gammaRbeta. An appropriate purified immunoglobulin isotype control is clone A19-3. A three-layer staining protocol is recommended for maximizing the detection IFN-gammaRbeta chains expressed by cells as detailed in the figure legend.
The IFN-gammaRbeta receptor epitope recognized by the MOB-47 antibody is blocked by bound IFN-gamma. Thus, it has been reported that cells which have bound IFN-gamma can be pretreated with dilute acid to remove the bound ligand before they are stained. IP/WB: The MOB-47 antibody has been reported to be useful for the immunoprecipitation and Western blotting of IFN-gammaRbeta chains from lysates of cloned mouse T cells.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Supplier Images
Flow Cytometry (FACS) image for anti-IFN-gamma Receptor (Chain beta) antibody (ABIN967655) Expression of cell surface IFN-gammaRbeta chains by BALB/c splenic lymphocytes (first...
 image for anti-IFN-gamma Receptor (Chain beta) antibody (ABIN967655) Histogram: Viable tumor cells
Product cited in: Bach, Szabo, Dighe, Ashkenazi, Aguet, Murphy, Schreiber: "Ligand-induced autoregulation of IFN-gamma receptor beta chain expression in T helper cell subsets." in: Science (New York, N.Y.), Vol. 270, Issue 5239, pp. 1215-8, 1996 (PubMed).