Images for product: anti-IFN-gamma Receptor (Chain beta) antibody
Expression of cell surface IFN-gammaRbeta chains by BALB/c splenic lymphocytes (first panel) and mouse A20 cells (second panel). BALB/c spleen cells and A20 cells (mouse B cell lymphoma) were preincubated (~15 min., 4°C) with purified 2.4G2 antibody [rat anti-mouse CD16 (FcgammaIII)/CD32 (FcgammaII), 1 µg antibody/10e6 cells] to block nonspecific staining due to immunoglobulin Fc receptors. The cells were stained (45 min., 4°C) with purified MOB-47 antibody (either at 0.5 µg mAb/10e6 BALB/c cells or at 0.125 µg mAb/10e6 A20 cells, ABIN967655). After washing, the cells were incubated (30 min., 4°C) with a biotin-conjugated cocktail of mouse anti-hamster antibodies (Clones G70-204 + G94-56, 0.5 µg mAb cocktail/10e6 cells). Finally, the cells were washed and incubated with R-PE-conjugated streptavidin (0.015 µg PE-SA/10e6 cells). After washing, the cells were analyzed with a FACScan™ Flow Cytometer. The immunofluorescent staining patterns for splenic lymphocytes (first panel) and A20 cells (second panel) that were either not stained with MOB-47 (grey line) or were stained with purified MOB-47 (black line) followed by the 2nd and 3rd layer reagents. The overlapping histograms were generated from reanalyzed flow cytometric data files that were gated for cells that had the light-scattering characteristics of lymphocytes (first panel) or viable tumor cells (second panel).