Pancreatic and Duodenal Homeobox 1 (PDX1) antibody

Details for Product No. ABIN967683
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Antigen
Synonyms ipf1, PDX1, Ad4BP, ELP, ELP-3, Ftz-F1, Ftzf1, SF-1, SF1, Ad4BP/SF-1, Ad4bp, Sf-1, GSF, IDX-1, IPF1, IUF1, MODY4, PDX-1, STF-1, IPF-1, Ipf1, Mody4, pdx-1, Idx1, Stf1, zgc:100874, XlHbox-8, xlhbox8
Reactivity
Human
(103), (39), (27), (14), (14), (6), (3), (1), (1)
Host
Mouse
(94), (17), (5), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(6), (6), (6), (5), (5), (5), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Intracellular Flow Cytometry (ICFC), BioImaging (BI), Immunofluorescence (IF)
(75), (71), (32), (16), (13), (10), (7), (6), (2), (2), (1), (1), (1), (1)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN967683
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Immunogen Human PDX-1 Recombinant Protein
Clone 658A5
Isotype IgG1, kappa
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Triton is a trademark of the Dow Chemical Company.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PDX-1
Background The 658A5 monoclonal antibody binds PDX-1 or Pancreas/Duodenum Homeobox Protein-1 encoded by the PDX1 or IPF1 (Insulin Promoter Factor 1) gene. PDX-1 is a key regulator of pancreatic development and adult beta-cell function. Loss of PDX1 gene function in mice and humans results in pancreatic agenesis. PDX1 is also involved in endocrine precursor cell development by binding the DNA-binding transcription factor Neurogenin 3. PDX-1 positive cells have been obtained by the directed differentiation of human embryonic stem cells through a definitive endoderm lineage.
Synonyms: PDX1, GSF, IPF-1, IPF1, IUF-1, IDX-1, STF-1
Molecular Weight 40 kDa
Gene ID 3651
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) antibody Western Blot analysis of PDX-1 in mouse pancreatic tumor (insulinoma) cell lysate. Lysate from Beta-TC-6 cells (ATCC CRL-11506™) was probed with Purified Mouse anti-PDX-1 monoclonal antibody (ABIN967683) at titrations of 0.125 (lane 1), 0.06 (lane 2), and 0.03 myg/ml (lane 3). PDX-1 is identified as a band of 40 kDa.
anti-Pancreatic and Duodenal Homeobox 1 (PDX1) antibody (2) Immunoflourescent staining of PDX-1 in mouse pancreatic tumor (insulinoma) cells. Beta-T-C6 cells (ATCC, CRL-11506™) were fixed with BD Cytofix™ Fixation Buffer, permeabilized with 0.1% Triton™ X-100 Buffer, and stained with Purified Mouse anti-PDX-1 monoclonal antibody (ABIN967683, pseudo-colored green) at 2.5 µg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and counter-staining was with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD AttoVision™ Software.
Product cited in: Ohlsson, Karlsson, Edlund: "IPF1, a homeodomain-containing transactivator of the insulin gene." in: The EMBO journal, Vol. 12, Issue 11, pp. 4251-9, 1993 (PubMed).

Gu, Dubauskaite, Melton: "Direct evidence for the pancreatic lineage: NGN3+ cells are islet progenitors and are distinct from duct progenitors." in: Development (Cambridge, England), Vol. 129, Issue 10, pp. 2447-57, 2002 (PubMed).

DAmour, Bang, Eliazer et al.: "Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells." in: Nature biotechnology, Vol. 24, Issue 11, pp. 1392-401, 2006 (PubMed).

Babu, Deering, Mirmira: "A feat of metabolic proportions: Pdx1 orchestrates islet development and function in the maintenance of glucose homeostasis." in: Molecular genetics and metabolism, Vol. 92, Issue 1-2, pp. 43-55, 2007 (PubMed).

Gannon, Ables, Crawford et al.: "pdx-1 function is specifically required in embryonic beta cells to generate appropriate numbers of endocrine cell types and maintain glucose homeostasis." in: Developmental biology, Vol. 314, Issue 2, pp. 406-17, 2008 (PubMed).

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