Paxillin (PXN) (AA 1-557) antibody
|Synonyms||FLJ16691, Pax, AW108311, AW123232, CG18061, CG18576, CT40481, CT42454, DPaxillin, DPxn, DPxn37, Dpax, PDLP, DmelCG31794, CG31794, LOC397826, wu:fw71f12, PXN, DKFZp469J2119, LOC100220858|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
|5 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Human, Dog (Canine), Mouse (Murine), Rat (Rattus)|
|Description||A number of cytoskeletal proteins are tyrosine phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts. One of these is the 68 kDa paxillin protein. Paxillin is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers. It is also present in the focal adhesions of Madin-Darby canine kidney epithelial cells, but is absent from the cell adherens junctions of these cells. Paxillin purified from chicken gizzard migrates as a diffuse band on SDS-PAGE with molecular weight of 65-70 kDa. It binds to the rod domain of vinculin, another focal adhesion protein. It is thought that phosphorylation of paxillin may have a role in that disassembly of focal adhesions and stress fibers during transformation. This antibody is routinely tested by Western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||68 kDa|
Related Products: ABIN968533, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Research Area||Cancer, Extracellular Matrix|
|Restrictions||For Research Use only|
|Western blot analysis of Paxillin on a A431 lysate. Lane 1: 1:10,000, lane 2: 1:20,000, lane 3: 1:40,000 dilution of the anti- Paxillin antibody. Immunofluoresence staining of human fibroblasts.|
Glenney, Zokas: "Novel tyrosine kinase substrates from Rous sarcoma virus-transformed cells are present in the membrane skeleton." in: The Journal of cell biology, Vol. 108, Issue 6, pp. 2401-8, 1989 (PubMed).
Wine, Chapin: "Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat." in: Journal of andrology, Vol. 20, Issue 2, pp. 198-213, 1999 (PubMed).
Nikolopoulos, Turner: "Actopaxin, a new focal adhesion protein that binds paxillin LD motifs and actin and regulates cell adhesion." in: The Journal of cell biology, Vol. 151, Issue 7, pp. 1435-48, 2001 (PubMed).
Even-Ram, Maoz, Pokroy et al.: "Tumor cell invasion is promoted by activation of protease activated receptor-1 in cooperation with the alpha vbeta 5 integrin." in: The Journal of biological chemistry, Vol. 276, Issue 14, pp. 10952-62, 2001 (PubMed).
van de Water, Houtepen, Huigsloot et al.: "Suppression of chemically induced apoptosis but not necrosis of renal proximal tubular epithelial (LLC-PK1) cells by focal adhesion kinase (FAK). Role of FAK in maintaining focal adhesion organization after acute renal cell injury." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36183-93, 2001 (PubMed).