MEK2 antibody (AA 1-110)

Catalog No. ABIN967846

This Mouse Monoclonal antibody specifically detects MEK2 in WB, IP, IF and IHC (f). It exhibits reactivity toward Human, Mouse, Rat, Dog, Chicken and Frog and has been mentioned in 5+ publications.

Quick Overview for MEK2 antibody (AA 1-110) (ABIN967846)

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Reactivity: Human, Mouse, Rat, Dog, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 96-MEK2

Product Details anti-MEK2 Antibody

Binding Specificity: AA 1-110

Cross-Reactivity: Mouse (Murine), Chicken, Dog (Canine), Frog, Human

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat MEK2 aa. 1-110

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-MEK2 antibody (ABIN967846)

Tworkowski, Salghetti, Tansey: "Stable and unstable pools of Myc protein exist in human cells." in: Oncogene, Vol. 21, Issue 55, pp. 8515-20, (2002) (PubMed).

Downey, Butler, Tapper, Fialkow, Saltiel, Rubin, Grinstein: "Importance of MEK in neutrophil microbicidal responsiveness." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 1, pp. 434-43, (1998) (PubMed).

Wu, Harrison, Dent, Lynch, Weber, Sturgill: "Identification and characterization of a new mammalian mitogen-activated protein kinase kinase, MKK2." in: Molecular and cellular biology, Vol. 13, Issue 8, pp. 4539-48, (1993) (PubMed).

Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, (1992) (PubMed).

Hattori, Fukuda, Yamashita, Nakamura, Gotoh, Nishida: "Activation of mitogen-activated protein kinase and its activator by ras in intact cells and in a cell-free system." in: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20346-51, (1992) (PubMed).

Target Details for MEK2

Target: MEK2 (MAP2K2) (Mitogen-Activated Protein Kinase Kinase 2 (MAP2K2))

Alternative Name: MEK2

Background: MEK2 (MAP Kinase Kinase 2 or ERK Kinase), a 46 kDa protein kinase, phosphorylates MAP kinases (ERKs) at tyrosine and threonine residues. This phosphorylation results in activation of the MAP kinases. MEK2 is seven amino acids larger and shares 81% identity with MEK1. In cultured cells, MEK2 is activated by serum. In vitro, v-Raf phosphorylates and activates MEK2. It is thought that all of these activated protein kinases are downstream of the Ras signal transduction pathway and represent an integral part of the Ras mitogenic signal. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase Kinase 2, ERK Kinase

Molecular Weight: 46 kDa

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling