CAN antibody (AA 247-449)

Catalog No. ABIN967861

Product Details anti-CAN Antibody

Target: CAN (Calcineurin (CAN))

Binding Specificity: AA 247-449

Reactivity: Human, Mouse, Rat, Chicken, Frog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Mouse (Murine), Chicken, Frog

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Calcineurin aa. 247-449

Clone: 29-Calcineurin

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-CAN antibody (ABIN967861)

Vega, Rothermel, Weinheimer, Kovacs, Naseem, Bassel-Duby, Williams, Olson: "Dual roles of modulatory calcineurin-interacting protein 1 in cardiac hypertrophy." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 2, pp. 669-74, (2003) (PubMed).

Jicha, Weaver, Lane, Vianna, Kress, Rockwood, Davies: "cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 17, pp. 7486-94, (1999) (PubMed).

Liang, Venema, Wang, Ju, Venema, Marrero: "Regulation of angiotensin II-induced phosphorylation of STAT3 in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 274, Issue 28, pp. 19846-51, (1999) (PubMed).

Sík, Hájos, Gulácsi, Mody, Freund: "The absence of a major Ca2+ signaling pathway in GABAergic neurons of the hippocampus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 6, pp. 3245-50, (1998) (PubMed).

Verin, Cooke, Herenyiova, Patterson, Garcia: "Role of Ca2+/calmodulin-dependent phosphatase 2B in thrombin-induced endothelial cell contractile responses." in: The American journal of physiology, Vol. 275, Issue 4 Pt 1, pp. L788-99, (1998) (PubMed).

Target Details for CAN

Target: CAN (Calcineurin (CAN))

Alternative Name: Calcineurin (CAN Products)

Synonyms: CALN antibody, CALNA antibody, CALNA1 antibody, CCN1 antibody, CNA1 antibody, PPP2B antibody, CAN A1/PP2B antibody, CG1455 antibody, Can antibody, Cana1 antibody, CnA antibody, CnnA21EF antibody, D14 antibody, DmCanA1-100B antibody, Dmel\\CG1455 antibody, PP2B antibody, PP2B 21EF antibody, PpD14 antibody, canA antibody, pMY4 antibody, 2900074D19Rik antibody, AI841391 antibody, AW413465 antibody, CN antibody, Caln antibody, Calna antibody, Calna1 antibody, protein phosphatase 3 catalytic subunit alpha antibody, Calcineurin A1 antibody, protein phosphatase 3, catalytic subunit, alpha isoform antibody, PPP3CA antibody, CanA1 antibody, Ppp3ca antibody

Background: Calcineurin is a Ca2+/calmodulin-dependent protein phosphatase. The active enzyme is a heterodimer of a large calmodulin-binding catalytic subunit A (61 kDa) and a smaller Ca2+ binding subunit B (19 kDa). Regions corresponding to the calmodulin-binding site, an autoinhibitory domain, and a putative subunit B binding site have been identified within the large subunit A. The activity of calcineurin is sensitive to immunosuppressants such as cyclosporin A (CsA) and tacrolimus (FK506). The study of FK506-mediated inhibition of nitric oxide formation has revealed that nitric oxide synthase (NOS) is a calcineurin substrate. Calcineurin dephosphorylates NOS and enhances its catalytic activity. Therefore, Calcineurin is an essential mediator for efficient T cell antigen receptor (TCR)-mediated T cell activation.

Molecular Weight: 61 kDa