CAN antibody (small, Subunit B)

Catalog No. ABIN2443839

The Mouse Monoclonal anti-CAN antibody (Clone VA1) (ABIN2443839) specifically detects CAN in WB, IHC, IP, ELISA and ICC. The antibody is reactive with Rat, Cow and Rabbit samples.

Quick Overview for CAN antibody (small, Subunit B) (ABIN2443839)

Target: CAN (Calcineurin (CAN))

Reactivity: Rat, Cow, Rabbit

Host: Mouse

Clonality: Monoclonal

Conjugate: This CAN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), ELISA, Immunocytochemistry (ICC)

Clone: VA1

Product Details anti-CAN Antibody

Binding Specificity: small, Subunit B

Purpose: Calcineurin Mouse Monoclonal Antibody (VA1)

Specificity: The antibody recognizes the regulatory β-subunit of calcineurin

Purification: Protein G purified

Immunogen: Full lenght bovine protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: If reconstituted with deionized water in 100 μL: WB: 1:2000, ICC: 1:200. Optimal dilution has to be determined by the user.

Buffer: Lyophilized protein G purified in PBS pH 7.4

Preservative: Without preservative

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Lyophilized antibodies can be kept at 4°C for up to 3 months and should be kept at -20°C for long-term storage (2 years). To avoid freeze-thaw cycles, reconstituted antibodies should be aliquoted before freezing for long-term (1 year) storage (-80°C) or kept at 4°C for short-term usage (2 months). For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made with the assay buffer. After the maximum long-term storage period (2 years lyophilized or 1 year reconstituted) antibodies should be tested in your assay with a standard sample to verify if you have noticed any decrease in their efficacy. To limit antibody loss or degradation, BSA (final concentration 1%) and sodium azide (final concentration 0.02%) can be added to the suggested first dilution. It is important to first verify if those preservatives are compatible with your assay.

Expiry Date: 24 months

Target Details for CAN

Target: CAN (Calcineurin (CAN))

Alternative Name: Calcineurin

Background:

Calcineurin beta-subunit

Within the last 30 years of research the biological roles of calcineurin have progressed from a putative inhibitor of the calmodulin dependent phosphodiesterase to the ground-breaking discovery that it is the target of immunosuppressant drugs such as cyclosporin A. As a serine/threonine protein phosphatase, calcineurin participates in a number of cellular processes and Ca2+ dependent signal transduction pathways. Calcineurin plays a key role in regulating the transcription factor NF-AT during T-cell activation, and in mediating responses of microorganisms to cation stress. Recently, this protein was studied extensively in relation to diseases such as schizophrenia and diabetes.

UniProt: P63099