Desmoglein (AA 705-1029) antibody

Catalog No. ABIN967873

Product Details

Target: Desmoglein

Binding Specificity: AA 705-1029

Reactivity: Blow Fly, Chicken, Dog, Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Western Blotting (WB)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken, Fruit Fly (Drosophila melanogaster)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Desmoglein aa. 705-1029

Clone: 62-Desmoglein

Isotype: IgG2b

Application Details

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Stewart, Barth, Nelson: "Differential regulation of endogenous cadherin expression in Madin-Darby canine kidney cells by cell-cell adhesion and activation of beta -catenin signaling." in: The Journal of biological chemistry, Vol. 275, Issue 27, pp. 20707-16, (2000) (PubMed).

Peltonen, Hentula, Hägg, Ylä-Outinen, Tuukkanen, Lakkakorpi, Rehn, Pihlajaniemi, Peltonen: "A novel component of epidermal cell-matrix and cell-cell contacts: transmembrane protein type XIII collagen." in: The Journal of investigative dermatology, Vol. 113, Issue 4, pp. 635-42, (1999) (PubMed).

Wine, Chapin: "Adhesion and signaling proteins spatiotemporally associated with spermiation in the rat." in: Journal of andrology, Vol. 20, Issue 2, pp. 198-213, (1999) (PubMed).

Mathur, Goodwin, Cowin: "Interactions of the cytoplasmic domain of the desmosomal cadherin Dsg1 with plakoglobin." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 14075-80, (1994) (PubMed).

Wheeler, Parker, Thomas, Ataliotis, Poynter, Arnemann, Rutman, Pidsley, Watt, Rees: "Desmosomal glycoprotein DGI, a component of intercellular desmosome junctions, is related to the cadherin family of cell adhesion molecules." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 11, pp. 4796-800, (1991) (PubMed).

Target Details

Target: Desmoglein

Background: Desmoglein (Dsg1) is a transmembrane glycoprotein found in desmosome junctions. Desmosomes and adherens junctions are plasma membrane specializations involved in cell-cell adhesion. They serve as membrane anchorage domains for intermediate filaments and microfilaments, respectively. Actin microfilaments anchor at adherens junctions, which occur in many sizes, shapes, and positions. These junctions generally contain E- or N-Cadherin whose cytoplasmic region is associated with cytoplasmic proteins such as alpha- and beta- catenin, vinculin, alpha-actinin, and radixin. Intermediate filaments (IFs) are anchored at desmosomes which contain desmosome-specific cadherin family members, known as desmocollin (Dsc) and desmoglein. These proteins associate with cytoplasmic proteins such as desmoplakin and plakoglobin (gamma-Catenin). Plakoglobin is the only junctional protein found at both desmosomes and adherens junctions and, therefore, plays a crucial role in cell adhesion.
Synonyms: Dsg1

Molecular Weight: 165 kDa