Cyclin D3 (CCND3) (AA 127-292) antibody

Details for Product No. ABIN967879
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Antigen
Synonyms zgc:103502, CCND3, 9230106B05Rik, AA682053, AL024085, AW146355, C78795
Epitope
AA 127-292
(31), (10), (9), (6), (6), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(143), (66), (57), (26), (26), (26), (14), (3)
Host
Mouse
(93), (50)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(7), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
(100), (39), (37), (28), (20), (20), (19), (17), (11), (10), (5), (2), (1), (1)
Pubmed 5 references available
Quantity 50 μg
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Catalog No. ABIN967879
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Immunogen Human Cyclin D3
Clone 1
Isotype IgG2b
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Cyclin D3
Background Several classes of cyclins (A-E) have been described. These proteins act as regulatory subunits for cyclin-dependent kinases (cdks). The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. There are at least 3 different D-type cyclins whose relative levels vary among cell types. Cyclin D3 is known to associate with Cdk5 and weakly with Cdk2. Cdk4 is preferentially bound by Cyclin D1. However, in T cells which do not express Cyclin D1, Cdk4 associates with Cyclins D2 and D3. While bound to cdks, the D-type cyclins also associate with the polymerase-delta subunit, PCNA. Cyclin D3 has also been shown to bind pRb in vitro. This suggests that pRb may be an in vivo substrate of D-type cyclins. This antibody is routinely tested by western blot analysis.
Molecular Weight 33 kDa
Research Area Cancer, Cell Cycle
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Cyclin D3 (CCND3) (AA 127-292) antibody Immunofluorescent staining of Human Endothelial cells.
anti-Cyclin D3 (CCND3) (AA 127-292) antibody (2) Western blot analysis of Cyclin D3 on RSV-3T3 lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the Cyclin D3 antibody.
Product cited in: Motokura, Keyomarsi, Kronenberg et al.: "Cloning and characterization of human cyclin D3, a cDNA closely related in sequence to the PRAD1/cyclin D1 proto-oncogene." in: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20412-5, 1992 (PubMed).

Xiong, Menninger, Beach et al.: "Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins." in: Genomics, Vol. 13, Issue 3, pp. 575-84, 1992 (PubMed).

Bagui, Jackson, Agrawal et al.: "Analysis of cyclin D3-cdk4 complexes in fibroblasts expressing and lacking p27(kip1) and p21(cip1)." in: Molecular and cellular biology, Vol. 20, Issue 23, pp. 8748-57, 2000 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Haller, Wu, Derow et al.: "Physical interaction of human T-cell leukemia virus type 1 Tax with cyclin-dependent kinase 4 stimulates the phosphorylation of retinoblastoma protein." in: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3327-38, 2002 (PubMed).

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