Jun Proto-Oncogene (JUN) (AA 26-175) antibody
|Synonyms||AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
|5 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Human, Chicken, Dog (Canine), Rat (Rattus)|
|Description||The activator protein transcription factor (AP-1) was identified as a protein that recognizes specific sequences in the cis-control regions of the SV40 virus and the human metallothionein IIA gene. AP-1 is composed of protein products of two different gene families: jun and fos. The AP-1 transcription factor is either a homodimer of Jun proteins or a heterodimer of Jun and Fos proteins. The transcriptional activity of Jun is enhanced by phosphorylation in its activation domain at Ser63 and Ser73. Phosphorylation at both sites is necessary for stimulating the activating function of Jun. Jun is phosphorylated by JNK protein kinases that are activated by the same signals that potentiate Jun activity. This antibody is routinely tested by western blot analysis.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||39 kDa|
Related Products: ABIN968536, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Restrictions||For Research Use only|
Lamph, Wamsley, Sassone-Corsi et al.: "Induction of proto-oncogene JUN/AP-1 by serum and TPA." in: Nature, Vol. 334, Issue 6183, pp. 629-31, 1988 (PubMed).
Taupin, Wu, Jeon et al.: "The trefoil gene family are coordinately expressed immediate-early genes: EGF receptor- and MAP kinase-dependent interregulation." in: The Journal of clinical investigation, Vol. 103, Issue 9, pp. R31-8, 1999 (PubMed).
Chang, Nakajima, Illenye et al.: "Caspase-dependent apoptosis by ectopic expression of E2F-4." in: Oncogene, Vol. 19, Issue 41, pp. 4713-20, 2000 (PubMed).
He, Stepulak, Holmström et al.: "The intermediate filament protein keratin 8 is a novel cytoplasmic substrate for c-Jun N-terminal kinase." in: The Journal of biological chemistry, Vol. 277, Issue 13, pp. 10767-74, 2002 (PubMed).
Doray, Ghosh, Griffith et al.: "Cooperation of GGAs and AP-1 in packaging MPRs at the trans-Golgi network." in: Science (New York, N.Y.), Vol. 297, Issue 5587, pp. 1700-3, 2002 (PubMed).