Protein Kinase C, delta (PKCd) (AA 114-289) antibody
|Synonyms||MAY1, PKCD, MGC49908, nPKC-delta, Pkcd, PKC[d], AI385711, PKCdelta, D14Ertd420e, fb09d10, fv43b11, zgc:56558, wu:fb09d10, wu:fv43b11, MGC115071, PKC-delta, pkc-delta2, PRKCD, may1, npkc-delta|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|5 references available|
|Quantity||50 µg (250 µg/ml) (Variants)|
|Price||Product not available in this region.|
|Alternative name||PKC delta|
|Cross-Reactivity||Rat (Rattus), Mouse (Murine)|
|Description||The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. PKCdelta is involved in myeloid differentiation, as well as in the secretory response of antigen-stimulated rat basophilic RBL 2H3 cells. Overexpression and subsequent stimulation of PKCdelta leads to cell cycle arrest in CHO cells and complete growth inhibition of NIH 3T3 cells. PKCdelta is the most abundant isoform in hemopoietic cells and is highly expressed in many other organs and tissues. This suggests that PKCdelta may be one of the major PKC isozymes in mammalian cells.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||78 kDa|
Related Products: ABIN968545
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20° C.|
|Research Area||Cancer, Signaling, Protein Modifications, Kinases/Phosphatases|
|Restrictions||For Research Use only|
Bell, Burns: "Lipid activation of protein kinase C." in: The Journal of biological chemistry, Vol. 266, Issue 8, pp. 4661-4, 1991 (PubMed).
Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, 1988 (PubMed).
Blass, Kronfeld, Kazimirsky et al.: "Tyrosine phosphorylation of protein kinase Cdelta is essential for its apoptotic effect in response to etoposide." in: Molecular and cellular biology, Vol. 22, Issue 1, pp. 182-95, 2001 (PubMed).
Ringshausen, Schneller, Bogner et al.: "Constitutively activated phosphatidylinositol-3 kinase (PI-3K) is involved in the defect of apoptosis in B-CLL: association with protein kinase Cdelta." in: Blood, Vol. 100, Issue 10, pp. 3741-8, 2002 (PubMed).
Larsen, Ueyama, Brannock et al.: "A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells." in: The Journal of cell biology, Vol. 159, Issue 6, pp. 939-44, 2002 (PubMed).
|Hosts||Rabbit (56), Mouse (2)|
|Reactivities||Human (54), Mouse (Murine) (37), Rat (Rattus) (36), Cow (Bovine) (2), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1)|
|Applications||Western Blotting (WB) (53), Immunohistochemistry (IHC) (34), ELISA (31), Immunofluorescence (IF) (13), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Immunocytochemistry (ICC) (5), Flow Cytometry (FACS) (3), Immunoprecipitation (IP) (2)|
|Conjugates||Alexa Flour 488 (1), Biotin (1)|
|Epitopes||pSer645 (8), N-Term (3), pTyr64,N-Term (3), Ser645 (2), pThr505 (1), pThr507 (1), pTyr313 (1), pTyr52 (1), pTyr64 (1)|