PKC delta antibody (AA 114-289)

Catalog No. ABIN967942

Product Details anti-PKC delta Antibody

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Binding Specificity: AA 114-289

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC delta antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Cross-Reactivity: Rat (Rattus), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PKCdelta aa. 114-289

Clone: 14-PKC delta

Isotype: IgG2b

Application Details

Comment:

Related Products: ABIN968545

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-PKC delta antibody (ABIN967942)

Larsen, Ueyama, Brannock, Shirai, Saito, Larsson, Loegering, Weber, Lennartz: "A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells." in: The Journal of cell biology, Vol. 159, Issue 6, pp. 939-44, (2002) (PubMed).

Ringshausen, Schneller, Bogner, Hipp, Duyster, Peschel, Decker: "Constitutively activated phosphatidylinositol-3 kinase (PI-3K) is involved in the defect of apoptosis in B-CLL: association with protein kinase Cdelta." in: Blood, Vol. 100, Issue 10, pp. 3741-8, (2002) (PubMed).

Blass, Kronfeld, Kazimirsky, Blumberg, Brodie: "Tyrosine phosphorylation of protein kinase Cdelta is essential for its apoptotic effect in response to etoposide." in: Molecular and cellular biology, Vol. 22, Issue 1, pp. 182-95, (2001) (PubMed).

Bell, Burns: "Lipid activation of protein kinase C." in: The Journal of biological chemistry, Vol. 266, Issue 8, pp. 4661-4, (1991) (PubMed).

Nishizuka: "The molecular heterogeneity of protein kinase C and its implications for cellular regulation." in: Nature, Vol. 334, Issue 6184, pp. 661-5, (1988) (PubMed).

Target Details for PKC delta

Target: PKC delta (PKCd) (Protein Kinase C, delta (PKCd))

Alternative Name: PKC delta (PKCd Products)

Synonyms: MAY1 antibody, PKCD antibody, nPKC-delta antibody, AI385711 antibody, D14Ertd420e antibody, PKC[d] antibody, PKCdelta antibody, Pkcd antibody, fb09d10 antibody, fv43b11 antibody, prkcd antibody, wu:fb09d10 antibody, wu:fv43b11 antibody, zgc:56558 antibody, PKC-delta antibody, may1 antibody, npkc-delta antibody, pkc-delta2 antibody, pkcd antibody, tpa-1 antibody, protein kinase C delta antibody, protein kinase C, delta antibody, protein kinase C, delta a antibody, protein kinase C delta L homeolog antibody, PRKCD antibody, Prkcd antibody, prkcda antibody, prkcd.L antibody, prkcd antibody

Background: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members (delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, iota, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. PKCdelta is involved in myeloid differentiation, as well as in the secretory response of antigen-stimulated rat basophilic RBL 2H3 cells. Overexpression and subsequent stimulation of PKCdelta leads to cell cycle arrest in CHO cells and complete growth inhibition of NIH 3T3 cells. PKCdelta is the most abundant isoform in hemopoietic cells and is highly expressed in many other organs and tissues. This suggests that PKCdelta may be one of the major PKC isozymes in mammalian cells.

Molecular Weight: 78 kDa

Pathways: Interferon-gamma Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, M Phase, G-protein mediated Events, Dicarboxylic Acid Transport, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, Lipid Metabolism