Integrin beta 1 (ITGB1) (AA 76-256) antibody

Antigen: Integrin beta 1 (ITGB1)

Synonyms: CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, VLA-BETA, Fnrb, gpIIa, AA409975, AA960159, 4633401G24Rik, ITGB1, MGC137337, itgb1-B

Binding Site: AA 76-256

Clonality: Monoclonal (18)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Catalog no.: ABIN967991

Additional Information

Alternative name: CD29

Immunogen: Human CD29

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 18

Description: Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. All integrins are transmembrane heterodimers composed of alpha and beta subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of 12 alpha and 9 beta subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The beta1 subgroup of this receptor family comprises at least six different dimer combinations. Among these combinations, alpha2beta1 is associated with type I collagen and laminin binding and regulation, alpha3beta1 is a receptor for laminin and epiligrin, and alpha5beta1 is a fibronectin receptor. beta1 integrins play an important role in several aspects of epidermal differentiation and morphogenesis. Expression of the beta1 subunit is regulated by growth factors such as TGF-beta1. Integrin activation, which enhances binding of T cells to endothelium, is regulated in part by phosphatidylinositol 3-kinase. Focal adhesion kinase (FAK) and paxillin have been reported to independently bind the C-terminal, cytoplasmic domain of the beta1 subunit. FAK is reported to be enzymatically activated upon engagement of integrins with their ligands and paxillin is phosphorylated on tyrosine upon activation of FAK. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin beta1, platelet GPIIa, VLA beta-chain

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 130 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Images

Western blot analysis of CD29 (Integrin beta1) on A431 lysate (Human epithelial carcinoma, ATCC CRL-1555). Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1: 10,000 dilution of the anti-CD29 antibody.

Immunofluorescence staining of HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2.2).

Publications

Product: Cervella, Silengo, Pastore et al.: "Human beta 1-integrin gene expression is regulated by two promoter regions." in: The Journal of biological chemistry, Vol. 268, Issue 7, pp. 5148-55, 1993 (PubMed).

Yeh, Peng, Huang: "Accutin, a new disintegrin, inhibits angiogenesis in vitro and in vivo by acting as integrin alphavbeta3 antagonist and inducing apoptosis." in: Blood, Vol. 92, Issue 9, pp. 3268-76, 1998 (PubMed).

Huan, van Adelsberg: "Polycystin-1, the PKD1 gene product, is in a complex containing E-cadherin and the catenins." in: The Journal of clinical investigation, Vol. 104, Issue 10, pp. 1459-68, 1999 (PubMed).

Tang, Hao, Fitzgerald et al.: "Pyk2/CAKbeta tyrosine kinase activity-mediated angiogenesis of pulmonary vascular endothelial cells." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 5441-7, 2002 (PubMed).

Ivaska, Whelan, Watson et al.: "PKC epsilon controls the traffic of beta1 integrins in motile cells." in: The EMBO journal, Vol. 21, Issue 14, pp. 3608-19, 2002 (PubMed).