Discs, Large Homolog 4 (Drosophila) (DLG4) (AA 353-504) antibody

Antigen: Discs, Large Homolog 4 (Drosophila) (DLG4)

Synonyms: PSD95, SAP90, SAP-90, FLJ97752, FLJ98574, DAP2, SAPAP2, Dlgh4, PSD-95, SAP90A, Sap90, 11, anon-EST:Posey93, anon-WO03040301.258, anon-WO03040301.260, anon-WO03040301.268, CG1725, CG1730, CPD, d. lg.-1, Discs-large, dlg, Dlg, DLG, dlg-1, dlg-A, Dlg-A, DLG-A, Dlg1, dlgA, DlgA, DmelCG1725, Drodlg, l(1)10Bf, l(1)bwn, l(1)d.lg-1, l(1)d.lg.-1, l(1)discs large, l(1)dlg, l(1)dlg-1, l(1)dlg1, l(1)G0276, l(1)G0342, l(1)G0456, l(1)G19, l(1)l.pr.-2, l(1)L11, l(1)lpr-2, misb, SAP97, Sapap2, 6430596N04Rik, dlgh4, psd95, sap90, sap-90, LLGL1

Binding Site: AA 353-504

Clonality: Monoclonal (16)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968003

Additional Information

Alternative name: PSD-95

Immunogen: Rat PSD-95

Cross-Reactivity: Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 16

Description: PostSynaptic Density (PSD) refers to a dense region of submembranous cytoskeleton found most prominently in postsynaptic membranes of the CNS. Possible functions for the PSD include regulation and aggregation of receptors, structural stabilization of the synaptic junction, and transduction of signals from membrane receptors. Some of the proteins associated with the PSD are fodrin, tubulin, calmodulin, CaM Kinase II, PSD-95, and PSD-93. PSD-95 (SAP90) is a protein that interacts with the NMDA receptor NMDAR2B, neuronal NOS (nNOS or bNOS), and other proteins. PSD-95 contains one SH3 domain in its carboxy-terminal domain, as well as three conserved repeat regions called GLGF or PDZ domains. nNOS, which is concentrated in synaptic junctions, also contains a PZD domain. PSD-95 and nNOS interact via their respective PZD domains, which may mediate the binding of nNOS to skeletal muscle syntrophin. This antibody is routinely tested by western blot analysis.
Synonyms: PostSynaptic Density-95, SAP90

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 95 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Synonyms: PSD95, SAP90, SAP-90, FLJ97752, FLJ98574, DAP2, SAPAP2, Dlgh4, PSD-95, SAP90A, Sap90, 11, anon-EST:Posey93, anon-WO03040301.258, anon-WO03040301.260, anon-WO03040301.268, CG1725, CG1730, CPD, d. lg.-1, Discs-large, dlg, Dlg, DLG, dlg-1, dlg-A, Dlg-A, DLG-A, Dlg1, dlgA, DlgA, DmelCG1725, Drodlg, l(1)10Bf, l(1)bwn, l(1)d.lg-1, l(1)d.lg.-1, l(1)discs large, l(1)dlg, l(1)dlg-1, l(1)dlg1, l(1)G0276, l(1)G0342, l(1)G0456, l(1)G19, l(1)l.pr.-2, l(1)L11, l(1)lpr-2, misb, SAP97, Sapap2, 6430596N04Rik, dlgh4, psd95, sap90, sap-90, LLGL1

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of PSD-95 on a rat cerebrum lysate. Lane1: 1:250, lane 2: 1: 500, lane 3: 1:1000 dilution of the mouse anti- PSD-95 antibody.

Immunofluorescence staining of PC12 cells (Rat neuroblastoma, ATCC CRL-1721).

anti-Discs, Large Homolog 4 (Drosophila) (DLG4) (AA 353-504) antibody (Image 3)

Publications

Product: Cho, Hunt, Kennedy: "The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein." in: Neuron, Vol. 9, Issue 5, pp. 929-42, 1992 (PubMed).

Brenman, Chao, Gee et al.: "Interaction of nitric oxide synthase with the postsynaptic density protein PSD-95 and alpha1-syntrophin mediated by PDZ domains." in: Cell, Vol. 84, Issue 5, pp. 757-67, 1996 (PubMed).

Mehta, Wu, Garner et al.: "Molecular mechanisms regulating the differential association of kainate receptor subunits with SAP90/PSD-95 and SAP97." in: The Journal of biological chemistry, Vol. 276, Issue 19, pp. 16092-9, 2001 (PubMed).

Fallon, Moreau, Croft et al.: "Parkin and CASK/LIN-2 associate via a PDZ-mediated interaction and are co-localized in lipid rafts and postsynaptic densities in brain." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 486-91, 2002 (PubMed).

Fagiolini, Katagiri, Miyamoto et al.: "Separable features of visual cortical plasticity revealed by N-methyl-D-aspartate receptor 2A signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, Issue 5, pp. 2854-9, 2003 (PubMed).