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Adaptin alpha (AA 38-215) antibody

Details for Product No. ABIN968008
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Antigen
Epitope
AA 38-215
Reactivity
Mouse (Murine)
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)
Pubmed 6 references available
Quantity 50 µg
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Catalog No. ABIN968008
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Immunogen Mouse Adaptin alpha [A]
Clone 8
Isotype IgG1
Cross-Reactivity Rat (Rattus), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (alpha, gamma, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain (sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. In the AP-2 complex, Adaptin alpha is expressed in two very similar isoforms from two different genes. Adaptin alpha [A] (112kDa) is expressed primarily in brain, while Adaptin alpha [C] (105kDa) is expressed in brain, liver and other tissues. This antibody is routinely tested by western blot analysis.
Molecular Weight 112 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Adaptin alpha (AA 38-215) antibody Western blot analysis of Adaptin alpha on a rat cerebrum lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the anti-Adaptin alpha antibody.
anti-Adaptin alpha (AA 38-215) antibody (2) Immunofluorescence staining of rat neurons.
Product cited in: Robinson: "Cloning of cDNAs encoding two related 100-kD coated vesicle proteins (alpha-adaptins)." in: The Journal of cell biology, Vol. 108, Issue 3, pp. 833-42, 1989 (PubMed).

Santolini, Puri, Salcini et al.: "Numb is an endocytic protein." in: The Journal of cell biology, Vol. 151, Issue 6, pp. 1345-52, 2001 (PubMed).

Teo, Tan, Lim et al.: "The tyrosine kinase ACK1 associates with clathrin-coated vesicles through a binding motif shared by arrestin and other adaptors." in: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 18392-8, 2001 (PubMed).

Wasiak, Legendre-Guillemin, Puertollano et al.: "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics." in: The Journal of cell biology, Vol. 158, Issue 5, pp. 855-62, 2002 (PubMed).

Jarousse, Wilson, Arac et al.: "Endocytosis of synaptotagmin 1 is mediated by a novel, tryptophan-containing motif." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 7, pp. 468-78, 2003 (PubMed).

General Jarousse, Kelly: "The AP2 binding site of synaptotagmin 1 is not an internalization signal but a regulator of endocytosis." in: The Journal of cell biology, Vol. 154, Issue 4, pp. 857-66, 2001 (PubMed).

Validation Images
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