alpha Adaptin antibody (AA 612-661)

Catalog No. ABIN6746729

The Rabbit Polyclonal anti-alpha Adaptin antibody is suitable to detect alpha Adaptin in samples from Human, Mouse, Rat, Horse, Rabbit, Guinea Pig and Monkey. It has been validated for WB.

Quick Overview for alpha Adaptin antibody (AA 612-661) (ABIN6746729)

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This alpha Adaptin antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-alpha Adaptin Antibody

Binding Specificity: AA 612-661

Specificity: Human AP2A1 / AP2-Alpha

Predicted Reactivity: Percent identity by BLAST analysis: Human, Horse (100%) Dog, Pig (92%) Mouse, Rat, Rabbit, Guinea pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa612-661 of human AP2A1 (O95782, NP_570603). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Monkey, Galago, Marmoset, Horse (100%), Dog, Bat, Pig (92%), Mouse, Rat, Rabbit, Guinea pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for alpha Adaptin

Target: alpha Adaptin (AP2A1) (Adaptor-Related Protein Complex 2, alpha 1 Subunit (AP2A1))

Alternative Name: AP2A1 / AP2-Alpha

Background: Name/Gene ID: AP2A1

Synonyms: AP2A1, Adaptin, alpha A, ADTAA, Alpha-adaptin A, Alpha1-adaptin, AP-2 complex subunit alpha-1, AP2 Alpha, CLAPA1, AP2-alpha

Gene ID: 160

NCBI Accession: NP_570603

UniProt: O95782

Pathways: Notch Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation