IQ Motif Containing GTPase Activating Protein 1 (IQGAP1) (AA 1348-1490) antibody

Antigen: IQ Motif Containing GTPase Activating Protein 1 (IQGAP1)

Synonyms: SAR1, p195, KIAA0051, HUMORFA01

Binding Site: AA 1348-1490

Clonality: Monoclonal (24)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN968063

Additional Information

Alternative name: IQGAP1

Immunogen: Human IQGAP1

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 24

Description: GTPases (Ras, Rho, cdc42Hs, and rac) modulate various cellular functions such as cytoskeletal architecture, growth, motility, and gene expression. The activity of the GTP-binding proteins is regulated by factors that accelerate GTP-binding (GAPs) and proteins that enhance the rate of GTP hydrolysis (GTPases). IQGAP1 is Ras-GAP related protein that contains a WW domain that binds poly-proline regions, a calponin-homology domain (CHD), an IQ domain of unconventional myosins, and a C-terminal GAP-related domain (GRD). IQGAP1 co-immunoprecipitates with cdc42 and calmodulin, and colocalizes with cdc42Hs at the leading edges of the plasma membrane and at the cell-cell junctions. IQGAP1 may interact with both cadherins and catenins leading to dissociation of these proteins from sites of cell adhesion. Thus, IQGAP1 may be a Rac1 and Cdc42 effector that negatively regulates cadherin-mediated cell junctions.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 195 kDa

Comments:

Related Products: ABIN968536, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cancer

Restrictions: For Research Use only

Images

Western blot analysis of IQGAP1 on a human endothelial cell lysate. Lane 1: 1:5000, lane 2: 1:10,000, lane 3: 1:20,000 dilution of the mouse anti-IQGAP1 antibody.

Immunofluorescence staining of WI-38 cells (Human lung fibroblasts, ATCC CCL-75).

Publications

Product: Hart, Callow, Souza et al.: "IQGAP1, a calmodulin-binding protein with a rasGAP-related domain, is a potential effector for cdc42Hs." in: The EMBO journal, Vol. 15, Issue 12, pp. 2997-3005, 1996 (PubMed).

Kuroda, Fukata, Kobayashi et al.: "Identification of IQGAP as a putative target for the small GTPases, Cdc42 and Rac1." in: The Journal of biological chemistry, Vol. 271, Issue 38, pp. 23363-7, 1996 (PubMed).

Li, Wang, Chakladar et al.: "Gastric hyperplasia in mice lacking the putative Cdc42 effector IQGAP1." in: Molecular and cellular biology, Vol. 20, Issue 2, pp. 697-701, 2000 (PubMed).

Ruiz-Velasco, Lanning, Williams: "The activation of Rac1 by M3 muscarinic acetylcholine receptors involves the translocation of Rac1 and IQGAP1 to cell junctions and changes in the composition of protein complexes containing Rac1, IQGAP1, and actin." in: The Journal of biological chemistry, Vol. 277, Issue 36, pp. 33081-91, 2002 (PubMed).

Mbele, Deloulme, Gentil et al.: "The zinc- and calcium-binding S100B interacts and co-localizes with IQGAP1 during dynamic rearrangement of cell membranes." in: The Journal of biological chemistry, Vol. 277, Issue 51, pp. 49998-50007, 2002 (PubMed).