IQGAP1 antibody

Catalog No. ABIN6719262

Product Details anti-IQGAP1 Antibody

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IQGAP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for IQGAP1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for IQGAP1 detection. Tested with WB, IHC-P, Direct ELISA in Human,Mouse,Rat.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human IQGAP1 recombinant protein (D1374-K1657).

Isotype: IgG

Application Details

Application Notes:

Application details: Western blot|0.1-0.5 μg/mL Immunohistochemistry(Paraffin-embedded Section)|0.5-1 μg/mL Direct ELISA|0.1-0.5 μg/mL

Comment:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for IQGAP1

Target: IQGAP1 (IQ Motif Containing GTPase Activating Protein 1 (IQGAP1))

Alternative Name: IQGAP1 (IQGAP1 Products)

Synonyms: HUMORFA01 antibody, SAR1 antibody, p195 antibody, AA682088 antibody, D7Ertd237e antibody, D7Ertd257e antibody, mKIAA0051 antibody, IQ motif containing GTPase activating protein 1 antibody, IQGAP1 antibody, Iqgap1 antibody

Background:

Synonyms: Ras GTPase-activating-like protein IQGAP1, p195, IQGAP1, KIAA0051

Background: Ras GTPase-activating-like protein IQGAP1 (IQGAP1) also known as p195 is a ubiquitously expressed protein that in humans is encoded by the IQGAP1 gene. It is mapped to 15q26.1. This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

Gene ID: 8826

UniProt: P46940

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2