ITGA5 antibody (AA 853-1002)
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- Target See all ITGA5 Antibodies
- ITGA5 (Integrin, alpha 5 (ITGA5))
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Binding Specificity
- AA 853-1002
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This ITGA5 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)
- Characteristics
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1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols. - Purification
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Immunogen
- Human Integrin alpha5 aa. 853-1002
- Clone
- 1-CD49e
- Isotype
- IgG2a
- Top Product
- Discover our top product ITGA5 Primary Antibody
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- Comment
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Related Products: ABIN968535, ABIN967389
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 250 μg/mL
- Buffer
- Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store undiluted at -20° C.
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Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3546-59, (2002) (PubMed).
: "Nischarin, a novel protein that interacts with the integrin alpha5 subunit and inhibits cell migration." in: The Journal of cell biology, Vol. 151, Issue 6, pp. 1141-54, (2001) (PubMed).
: "Anchorage-dependent regulation of the mitogen-activated protein kinase cascade by growth factors is supported by a variety of integrin alpha chains." in: The Journal of biological chemistry, Vol. 274, Issue 44, pp. 31223-8, (1999) (PubMed).
: "Functional role of N-glycosylation in alpha 5 beta 1 integrin receptor. De-N-glycosylation induces dissociation or altered association of alpha 5 and beta 1 subunits and concomitant loss of ..." in: The Journal of biological chemistry, Vol. 269, Issue 16, pp. 12325-31, (1994) (PubMed).
: "Functional role of the cytoplasmic domain of the integrin alpha 5 subunit." in: The Journal of cell biology, Vol. 122, Issue 1, pp. 209-21, (1993) (PubMed).
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Fibronectin polymerization regulates the composition and stability of extracellular matrix fibrils and cell-matrix adhesions." in: Molecular biology of the cell, Vol. 13, Issue 10, pp. 3546-59, (2002) (PubMed).
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- Target
- ITGA5 (Integrin, alpha 5 (ITGA5))
- Alternative Name
- CD49e (ITGA5 Products)
- Synonyms
- ITGA5 antibody, Cd49e antibody, Fnra antibody, VLA5 antibody, CD49e antibody, FNRA antibody, VLA5A antibody, Itga5_retired antibody, integrin subunit alpha 5 antibody, integrin alpha 5 (fibronectin receptor alpha) antibody, ITGA5 antibody, Itga5 antibody
- Background
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Integrins are membrane receptors that mediate cell-cell or cell-matrix adhesion. They are transmembrane heterodimers composed of alpha and beta subunits and are connected to the cytoskeleton. At least 20 integrins, formed from combinations of 12 alpha and 9 beta subunits, have been reported. Many of these have been implicated as transducers of molecular signals. The integrin alpha5 subunit is 1049 amino acids with a molecular weight of 150-165 kDa. alpha5 and beta1 form a functional fibronectin receptor which is important for vascular development in mice. Both subunits of the fibronectin receptor are heavily glycosylated in their extracellular domains. This modification is essential for proper cell attachment to basal membranes. Integrin alpha5 has been reported to suppress apoptosis by a Bcl-2 pathway and the C-terminal region is critical for cell motility and cytoskeletal rearrangements. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin alpha5 - Molecular Weight
- 150 kDa
- Pathways
- Integrin Complex
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