Immunofluorescent staining of SH-SY5Y cells (Second Panel). Cells were seeded in a collagen coated 384 well imaging plate at ~ 8,000 cells per well. After overnight incubation, cells were stained using the Triton X100 fix/perm protocol and the anti-Carboxypeptidase E antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen). The image was taken on a Pathway 855 or 435 imager using a 20x objective. This antibody also stained SK-N-SH and C6 cells using both the Triton X100 and methanol fix/perm protocols.