Cullin 2 (CUL2) (AA 18-182) antibody

Details for Product No. ABIN968173
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Antigen
Synonyms 1300003D18Rik, 4932411N15Rik, AI327301, mKIAA4106, ATCUL2, F22D16.2, F22D16_2, cullin 2, cul2, Cullin-2, fj48a06, wu:fj48a06, cullin-2, LOC100221330
Epitope
AA 18-182
(20), (5), (2), (2), (2), (1), (1), (1)
Reactivity
Human
(69), (37), (21), (14), (14), (1)
Host
Mouse
(65), (4), (3)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(53), (34), (22), (17), (14), (12), (10), (5), (2), (1), (1)
Pubmed 2 references available
Catalog no. ABIN968173
Quantity 50 µg
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Immunogen Human CUL-2
Clone 4
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name CUL-2
Background The cullins are a family of proteins that are integral to cell cycle regulation. Members of this family include human Cul-2, C.elegans Ce-Cul-1, and S. cerevisiae Cdc53. Proper control of cell cycle progression is essential for the prevention of tumorigenesis. Mutant forms of both Ce-Cul-1 and Cdc53 have been implicated in the oncogenic process. Abnormal nuclear localization of Cul-2 is seen in a rare hereditary condition known as VHL (von Hippel-Lindau) syndrome. Patients with VHL syndrome possess a mutant VHL gene and develop cancers such as retinal anginomas, CNS hemangioblastomas, and renal cell carcinomas. In normal cells, the VHL protein competes with subunit A of elongin, a transcription elongation factor, for binding to subunits B and C. The formation of the VHL-B-C complex inhibits elongation. Additional data indicates that this complex associates with CUL-2 and promotes CUL-2 nuclear translocation. In cells with mutated VHL, transcriptional elongation is not efficiently curtailed and CUL-2 is not properly localized in the nucleus. Therefore, CUL-2 mediates VHL function, suggesting CUL-2 may be a candidate human tumor suppressor. This antibody is routinely tested by western blot analysis.
Synonyms: Cullin-2
Molecular Weight 76 kDa
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Cullin 2 (CUL2) (AA 18-182) antibody Western blot analysis of CUL-2 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the mouse anti- CUL-2 antibody.
anti-Cullin 2 (CUL2) (AA 18-182) antibody (2) Immunofluorescence staining of human endothelial cells.
anti-Cullin 2 (CUL2) (AA 18-182) antibody (3) anti-Cullin 2 (CUL2) (AA 18-182) antibody (Image 3)
Product cited in: Pause, Lee, Worrell et al.: "The von Hippel-Lindau tumor-suppressor gene product forms a stable complex with human CUL-2, a member of the Cdc53 family of proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 6, pp. 2156-61, 1997 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Hosts (65), (4), (3)
Reactivities (69), (37), (21), (14), (14), (1)
Applications (53), (34), (22), (17), (14), (12), (10), (5), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (20), (5), (2), (2), (2), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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