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Details for Product No. ABIN968203

p62 Lck Ligand (AA 257-437) antibody

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Antigen
Epitope
AA 257-437
Reactivity
Human
Host
Mouse
Clonality (Clone) Monoclonal ()
Application
Western Blotting (WB), Immunofluorescence (IF)
Pubmed 4 references available
Catalog no. ABIN968203
Quantity 150 µg
Price
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Immunogen Human p62 lck ligand
Clone 3
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Background P62 lck ligand (zeta-interacting protein (ZIP)) is a cytoplasmic protein that binds to the SH2 domain of lck (a T cell src tyrosine kinase) in the absence of a phosphotyrosine in either protein. The ubiquitously expressed p62 lck ligand contains a cysteine rich region that is similar to a zinc finger domain, a G protein binding region, a PEST sequence, and several phosphorylation sites. Deletion of the p62 lck ligand N-terminal domain has been reported to abrogate its binding to lck. However, mutation of the tyrosine did not have an effect. In addition, p62 lck ligand binds to the pseudosubstrate region of the PKCzeta catalytic domain. In turn, PKCzeta phosphorylates p62. p62 lck ligand binds to the dimerization region of PKCzeta, thereby inhibiting PKCzeta-PKCzeta interaction. This suggests that p62 lck ligand may compete with PKCzeta. However, it requires PKCzeta for proper subcellular localization. These data suggest that p62 lck ligand may be part of the protein bridge that links PKCzeta to the tyrosine kinases involved in signaling pathways. This antibody is routinely tested by western blot analysis.
Synonyms: Zeta Interacting Protein (ZIP)
Molecular Weight 62 kDa
Comment

Related Products: ABIN968551, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Storage -20 °C
Product cited in: Joung, Strominger, Shin: "Molecular cloning of a phosphotyrosine-independent ligand of the p56lck SH2 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 12, pp. 5991-5, 1996 (PubMed).

Puls, Schmidt, Grawe et al.: "Interaction of protein kinase C zeta with ZIP, a novel protein kinase C-binding protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 12, pp. 6191-6, 1997 (PubMed).

Wooten, Seibenhener, Mamidipudi et al.: "The atypical protein kinase C-interacting protein p62 is a scaffold for NF-kappaB activation by nerve growth factor." in: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 7709-12, 2001 (PubMed).

Cariou, Perdereau, Cailliau et al.: "The adapter protein ZIP binds Grb14 and regulates its inhibitory action on insulin signaling by recruiting protein kinase Czeta." in: Molecular and cellular biology, Vol. 22, Issue 20, pp. 6959-70, 2002 (PubMed).

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