Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5) (AA 13-188) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 5 (MAP2K5)

Synonyms: MEK5, MAPKK5, PRKMK5, HsT17454, Mapkk5, Prkmk5, AI324775, AI428457, Mek5, map2k5, fb73f02, MGC172137, wu:fb73f02

Binding Site: AA 13-188

Clonality: Monoclonal (21)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968272

Additional Information

Alternative name: MEK5

Immunogen: Human MEK5

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 21

Description: The mitogen-activated protein (MAP) kinase cascade participates in the Ras signal transduction pathway. The MAP kinase cascade consists of MEK kinase (Raf-1 or B-Raf), MAP/ERK kinase (MEK1 or MEK2), and extracellular-regulated protein kinase (ERK1 or ERK2). Raf becomes activated following its interaction with Ras-GTP (activated Ras). Via phosphorylation reactions, Raf activates MEK which, in turn, activates ERK. MEK5, also known as MAP Kinase 5, is a 50 kDa protein that is 40% identical to MEK1 and MEK2. Alternative splicing results in two MEK5 isoforms: alpha (50 kDa) and beta (40 kDa). MEK5beta has been reported to be ubiquitously expressed while MEK5alpha is expressed primarily in the liver and brain. The N-terminal amino acid sequence of MEK5alpha resembles sequences found in actin cytoskeletal proteins, indicating that MEK5alpha may associate with the cytoskeleton. However, the role of MEK5 in the MAP kinase pathway and its substrate remain to be identified. This antibody is routinely tested by western blot analysis.
Synonyms: MAP Kinase-5

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 50 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Cancer, Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot analysis of MEK5 on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB-152). Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1:4000 dilution of the mouse anti- MEK5 antibody.

Immunofluorescence staining on human endothelial cells.

Publications

Product: English, Vanderbilt, Xu et al.: "Isolation of MEK5 and differential expression of alternatively spliced forms." in: The Journal of biological chemistry, Vol. 270, Issue 48, pp. 28897-902, 1996 (PubMed).

Zhou, Bao, Dixon: "Components of a new human protein kinase signal transduction pathway." in: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12665-9, 1995 (PubMed).