Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3) (AA 27-135) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3)

Synonyms: MEKK3, MAPKKK3, Mekk3, AW548911, KIAA4031, mKIAA4031

Binding Site: AA 27-135

Clonality: Monoclonal (40)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968365

Additional Information

Alternative name: MEKK3

Immunogen: Human MEKK3

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 40

Description: The Ras signaling pathway links signals from growth factor receptors to activation of the MEK/ERK kinases. This cascade of phosphorylation leads to cell growth and differentiation. External stimuli such as endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression. These processes are controlled by the MKK3/p38MAP kinase cascade. A third pathway responsive to environmental stress, cell death, or apoptosis is regulated by SEK/SAPKs. When overexpresssed, MEKK3 is a kinase that is capable of activating both the ERK and SAPK cascades. MEKK3 is 626 amino acids long and is ubiquitously expressed. It is positioned upstream of SEK and MEK in the signaling pathways and directly phosphorylates these enzymes. Overexpression of MEKK3 results in the activation of NFkappaB. Thus, MEKK3 represents an emerging family of kinases that is capable of inducing more than one signaling pathway. This indicates that specificity in the signaling cascades is probably achieved by effector kinases such as the ERKs and SAPKs.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 71 kDa

Comments:

Related Products: ABIN967389, ABIN968535

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Immunology, Innate Immunity, Kinases/Phosphatases

Restrictions: For Research Use only

Images

Western blot analysis of MEKK3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-MEKK3 antibody.

Immunofluorescence staining of RSV-3T3 cells.

Publications

Product: Ellinger-Ziegelbauer, Brown, Kelly et al.: "Direct activation of the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by an inducible mitogen-activated protein Kinase/ERK kinase kinase 3 (MEKK) derivative." in: The Journal of biological chemistry, Vol. 272, Issue 5, pp. 2668-74, 1997 (PubMed).

Yang, Lai, Lai et al.: "Phenethyl isothiocyanate inhibits migration and invasion of human gastric cancer AGS cells through suppressing MAPK and NF-kappaB signal pathways." in: Anticancer research, Vol. 30, Issue 6, pp. 2135-43, 2010 (PubMed).