NPAT antibody (AA 681-803)

Catalog No. ABIN968484

Product Details anti-NPAT Antibody

Target: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Binding Specificity: AA 681-803

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NPAT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human NPAT aa. 681-803

Clone: 27-NPAT

Isotype: IgG2b

Application Details

Comment:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-NPAT antibody (ABIN968484)

Zhao, Dynlacht, Imai, Hori, Harlow: "Expression of NPAT, a novel substrate of cyclin E-CDK2, promotes S-phase entry." in: Genes & development, Vol. 12, Issue 4, pp. 456-61, (1998) (PubMed).

Target Details for NPAT

Target: NPAT (Nuclear Protein, Ataxia-Telangiectasia Locus (NPAT))

Alternative Name: NPAT (NPAT Products)

Synonyms: NPAT-like antibody, im:6901964 antibody, DKFZp469B186 antibody, 6820401K01 antibody, AI427561 antibody, BB112559 antibody, E14 antibody, E14/NPAT antibody, p220 antibody, nuclear protein, co-activator of histone transcription antibody, nuclear protein, coactivator of histone transcription antibody, protein NPAT antibody, nuclear protein, ataxia-telangiectasia locus antibody, nuclear protein in the AT region antibody, nuclear protein, ataxia-telangiectasia locus S homeolog antibody, Npat antibody, NPAT antibody, LOC489420 antibody, npat antibody, LOC100286556 antibody, LOC100402032 antibody, LOC100480887 antibody, LOC100540521 antibody, LOC100561473 antibody, LOC100627195 antibody, npat.S antibody

Background: Cyclins regulate transitions between cell cycle phases by acting as regulatory subunits of the cyclin-dependent kinases (cdk). The temporal expression of cyclins is tightly regulated and plays a critical role in controlling the enzymatic activity of the cdks. Cyclin-dependent kinase 2 (Cdk2) is a member of a family of cdc2-related cell cycle protein kinases. Cdk2 is expressed earlier in the cell cycle than cdc2 and forms complexes with cyclins A, E, D1, and D3. It is not known if the D cyclins can form active complexes with Cdk2. Cyclin E-Cdk2 kinase is active in the G1 and S phases of the cell cycle and is important, as is Cyclin A-Cdk2, for the progression from G1 to S phase. One substrate for cyclin E-Cdk2 is a Nuclear Protein mapped to the Ataxia Telangiectasia locus, NPAT. This protein associates with cyclin E-Cdk2 and can be phosphorylated by Cdk2. NPAT protein levels peak at the G1/S boundary and overexpression of NPAT accelerates S phase entry, especially after coexpression of cyclin E-Cdk2. Thus, NPAT is a substrate of cyclin E-Cdk2 that may mediate G1 to S phase transition.
Synonyms: Nuclear Protein Ataxia Telangiectasia

Molecular Weight: 210 kDa