Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 3 (APBA3) (AA 63-185) antibody

Details for Product No. ABIN968505, Supplier: Log in to see
Antigen
  • AW208791
  • Mint-3
  • X11gamma
  • lin-10
  • mint3
  • MGC:15815
  • X11L2
  • Mint3
  • amyloid beta (A4) precursor protein-binding, family A, member 3
  • APBA3
  • Apba3
Epitope
AA 63-185
18
15
10
9
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
36
17
16
3
Host
Mouse
52
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
38
24
13
5
3
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Mouse Mint3 aa. 63-185
Clone 32-Mint3
Isotype IgG1
Cross-Reactivity Human
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Mint3 (APBA3 Antibody Abstract)
Background Munc18-1-interacting proteins, Mint1, Mint2, and Mint3, are members of the X11 family of proteins, which contain a phosphotyrosine-binding (PTB) domain and two PSD-95-DLG-ZO-1 (PDZ) domains. The PTB domain binds Asn-Pro-X-pTyr, a beta-turn motif found on activated growth factor receptors and other signaling molecules. PDZ domains bind the C-terminus of proteins involved in receptor and channel clustering and protein localization in polarized cells. Both Mint1 and Mint2 are expressed primarily in the nervous system and may interact with Munc18-1 and syntaxin to form a multimeric complex that mediates appropriate docking/fusion of synaptic vesicles. In addition, both Mint1 and Mint2 may be involved in Alzheimer's disease, since Mint2 colocalizes with amyloid precursor protein (APP) and is found in neuritic plaques, while Mint1 can bind APP, and inhibits the processing of APP to the amyloid beta peptide. Mint3 can also bind APP, but differs from Mint1 and Mint2 in its N-terminal region. It is also more widely expressed. Thus, Mint3 may function in signaling pathways, vesicle exocytosis, and/or protein targeting in a wide range of tissues. Mint3 has a calculated molecular weight of 61 kD, but reportedly is observed migrating at 86-89 kD. This antibody is routinely tested by western blot analysis.
Synonyms: X11gamma
Molecular Weight 86-89 kDa
Research Area Neurology, Alzheimer's Disease
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 3 (APBA3) (AA 63-185) antibody (ABIN968505) Western blot analysis of Mint3 on a RSV-3T3 cell lysate. Lane 1: 1:250, lane 2: 1:500...
Immunofluorescence (IF) image for anti-Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 3 (APBA3) (AA 63-185) antibody (ABIN968505) Immunofluorescence staining of WI-38 cells (human lung fibroblasts, ATCC CCL-75).
 image for anti-Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 3 (APBA3) (AA 63-185) antibody (ABIN968505) anti-Amyloid beta (A4) Precursor Protein-Binding, Family A, Member 3 (APBA3) (AA 63-185) antibody (Image 3)
Product cited in: McLoughlin, Irving, Brownlees, Brion, Leroy, Miller: "Mint2/X11-like colocalizes with the Alzheimer's disease amyloid precursor protein and is associated with neuritic plaques in Alzheimer's disease." in: The European journal of neuroscience, Vol. 11, Issue 6, pp. 1988-94, 1999 (PubMed).

Borg, Straight, Kaech, de Taddéo-Borg, Kroon, Karnak, Turner, Kim, Margolis: "Identification of an evolutionarily conserved heterotrimeric protein complex involved in protein targeting." in: The Journal of biological chemistry, Vol. 273, Issue 48, pp. 31633-6, 1998 (PubMed).

Borg, Yang, De Taddéo-Borg, Margolis, Turner: "The X11alpha protein slows cellular amyloid precursor protein processing and reduces Abeta40 and Abeta42 secretion." in: The Journal of biological chemistry, Vol. 273, Issue 24, pp. 14761-6, 1998 (PubMed).

Did you look for something else?