Dynamin Associated Protein 160 (DAP160) (AA 800-909) antibody

Catalog No. ABIN968598

Product Details

Target: Dynamin Associated Protein 160 (DAP160)

Binding Specificity: AA 800-909

Reactivity: Chicken, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunofluorescence (IF), Western Blotting (WB)

Cross-Reactivity: Rat (Rattus), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Ese-1 aa. 800-909

Clone: 29-Intersectin

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Okamoto, Schoch, Südhof: "EHSH1/intersectin, a protein that contains EH and SH3 domains and binds to dynamin and SNAP-25. A protein connection between exocytosis and endocytosis?" in: The Journal of biological chemistry, Vol. 274, Issue 26, pp. 18446-54, (1999) (PubMed).

Sengar, Wang, Bishay, Cohen, Egan: "The EH and SH3 domain Ese proteins regulate endocytosis by linking to dynamin and Eps15." in: The EMBO journal, Vol. 18, Issue 5, pp. 1159-71, (1999) (PubMed).

Target Details

Target: Dynamin Associated Protein 160 (DAP160)

Alternative Name: Intersectin

Background: ESE-1 (epithelium-specific, EH domain and SH3 domain regulator of endocytosis) contains two N-terminal EH (Ep15 homology) domains, a central coiled-coil domain, and five C-terminal SH3 domains. ESE-1 is also known as the adaptor protein Intersectin. Intersectin constitutively associates with Eps15 to form a complex that is analogous to the yeast Pan1p-End3p complex, which is required for endocytosis and organization of the actin cytoskeleton. The EH, coiled-coil, and SH3 domains of intersectin bind epsin family proteins, SNAP25 and SNAP23, and dynamin, respectively. Thus, intersectin functions in complex with Eps15 to regulate endocytosis by linking components of the endocytic machinery.

Molecular Weight: 140/200 kDa