Superoxide Dismutase 2, Mitochondrial (SOD2) (AA 114-220) antibody

Antigen: Superoxide Dismutase 2, Mitochondrial (SOD2)

Synonyms: MnSOD, Sod-2, MGC6144, LOC100101896, Sod2, MNSOD, IPO-B, MVCD6, Mn-SOD, MGC128371, cb463, zgc:73051, wu:fj33b01, GB14346, sod2, MGC88869, SOD2, DKFZp459E042, LOC100282741

Binding Site: AA 114-220

Clonality: Monoclonal (19)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968603

Additional Information

Alternative name: MnSOD

Immunogen: Mouse MnSOD

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 19

Description: Superoxide dismutase (SOD), catalase, and glutathione peroxidase are necessary for preventing the intracellular accumulation of reactive oxygen species. Three forms of SOD include the cytosolic Cu/Zn2+ SOD, the extracelluar Cu/Zn[2+] SOD, and the mitochondrial manganese (Mn) superoxide dismutase (MnSOD). In the mitochondria, MnSOD catalyzes the dismutation of two superoxide radicals, producing H2O2 and oxygen. Following protein synthesis, MnSOD is post-transcriptionally modified for transport from the cytosol into the mitochondria. In rat hepatocytes, H2O2 exposure induces MnSOD expression, while in rat lung, exposure to smoke induces expression in bronchial epithelial cells. In addition, MnSOD expression is required for PC12 cell resistance to nitric oxide (NO) toxicity and for nNOS neuron resistance to NMDA-induced NO toxicity. Superoxide radical anions may induce transcriptional activation of the MnSOD gene through PKC phosphorylation and activation of a CREB-1/ATF-1-like factor. Thus, MnSOD removal of reactive oxygen species created by exposure to toxic stimuli is thought to be critical for the survival of a variety of cell types. This antibody is routinely tested by western blot analysis.
Synonyms: Manganese Superoxide Dismutase

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 25 kDa

Comments:

Related Products: ABIN968542, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of MnSOD on a mouse kidney lysate. Lane 1: 1:1000, lane 2: 1:2000, lane 3: 1: 4000 dilution of the mouse anti- MnSOD antibody.

Publications

Product: Gilks, Price, Wright et al.: "Antioxidant gene expression in rat lung after exposure to cigarette smoke." in: The American journal of pathology, Vol. 152, Issue 1, pp. 269-78, 1998 (PubMed).

Röhrdanz, Kahl: "Alterations of antioxidant enzyme expression in response to hydrogen peroxide." in: Free radical biology & medicine, Vol. 24, Issue 1, pp. 27-38, 1998 (PubMed).

Gonzalez-Zulueta, Ensz, Mukhina et al.: "Manganese superoxide dismutase protects nNOS neurons from NMDA and nitric oxide-mediated neurotoxicity." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 18, Issue 6, pp. 2040-55, 1998 (PubMed).

Ambrosone, Freudenheim, Thompson et al.: "Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer." in: Cancer research, Vol. 59, Issue 3, pp. 602-6, 1999 (PubMed).

Kim, Roe, Chock et al.: "Transcriptional activation of the human manganese superoxide dismutase gene mediated by tetradecanoylphorbol acetate." in: The Journal of biological chemistry, Vol. 274, Issue 52, pp. 37455-60, 2000 (PubMed).