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Pericentrin (PCNT) (AA 1692-1814) antibody

Details for Product No. ABIN968665, Supplier: Login to see
Antigen
  • AKAP450
  • CG13459
  • CG18648
  • CG33957
  • CG6735
  • CP309
  • Cp309
  • D-PLP
  • DPLP
  • Dmel\\CG33957
  • Dplp
  • PACT
  • PLP
  • Plp
  • d-plp
  • dPLP
  • dplp
  • l(3)s2172
  • pPlp
  • KEN
  • MOPD2
  • PCN
  • PCNT2
  • PCNTB
  • PCTN2
  • SCKL4
  • DIP2A
  • AW476095
  • C86676
  • Pcnt2
  • kendrin
  • m239Asp
  • m275Asp
Epitope
AA 1692-1814
2
1
1
1
1
Reactivity
Mouse (Murine)
11
5
4
Host
Mouse
11
1
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
1
Application
Immunofluorescence (IF), Western Blotting (WB)
7
3
3
2
2
1
1
Supplier
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Immunogen Mouse pericentrin aa. 1692-1814
Clone 30-Pericentrin
Isotype IgG1
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Pericentrin (PCNT Antibody Abstract)
Background Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple alpha-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3 MDa-complex that includes gamma-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and gamma-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and gamma-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity. This antibody is routinely tested by western blot analysis.
Molecular Weight 220 kDa
Research Area Organelles
Pathways
Comment

Related Products: ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Pericentrin (PCNT) (AA 1692-1814) antibody (ABIN968665) Western blot analysis of Pericentrin on mouse neonate lysate. Lane 1: 1:500, lane 2: ...
Immunofluorescence (IF) image for anti-Pericentrin (PCNT) (AA 1692-1814) antibody (ABIN968665) Immunofluorescent staining on NIH-3T3 cells.
Product cited in: Young, Dictenberg, Purohit et al.: "Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes." in: Molecular biology of the cell, Vol. 11, Issue 6, pp. 2047-56, 2000 (PubMed).

Background publications Doxsey, Stein, Evans et al.: "Pericentrin, a highly conserved centrosome protein involved in microtubule organization." in: Cell, Vol. 76, Issue 4, pp. 639-50, 1994 (PubMed).