Pericentrin (PCNT) (AA 1692-1814) antibody

Antigen: Pericentrin (PCNT)

Synonyms: KEN, PCN, MOPD2, PCNT2, PCNTB, PCTN2, SCKL4, Pcnt2, C86676, kendrin, m239Asp, m275Asp, AW476095, DIP2A, AKAP450, CG13459, CG18648, CG33957, CG6735, CP309, d-plp, D-PLP, DmelCG33957, dplp, DPLP, l(3)s2172, PACT, Plp, pPlp

Binding Site: AA 1692-1814

Clonality: Monoclonal (30)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Catalog no.: ABIN968666

Additional Information

Alternative name: Pericentrin

Immunogen: Mouse pericentrin

Format: Liquid

Isotype: IgG1

Clone: 30

Description: Centrosomes and other microtubule-organizing centers are a diverse group of organelles that nucleate and organize microtubules for many celllular processes, such as mitotic spindle formation, organelle tranpsort, and protein localization. Pericentrin is a protein in the pericentriolar material, a filamentous matrix surrounding centrioles, which can organize microtubule spindle formation. The structure of pericentrin includes multiple alpha-helical domains that form coiled-coil domains separated by non-helical, non-coiled regions. It is found in a 3 MDa-complex that includes gamma-tubulin, a form of tubulin that nucleates microtubule formation at the centrosome. Pericentrin exhibits the highest expression in embryonic mouse kidney, thymus, and liver. Injection of pericentrin antibodies in Xenopus oocytes disrupts mitotic and meiotic divisions and blocks microtubule aster formation. In addition, dynein transports both pericentrin and gamma-tubulin to centrosomes along microtubules in Xenopus oocyte extracts. Thus, the complex of pericentrin and gamma-tubulin may be recruited to the centrosome by dynein, where the complex becomes anchored to the centrosome for microtubule nucleating activity. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 220 kDa

Comments:

Related Products: ABIN967389

Synonyms: KEN, PCN, MOPD2, PCNT2, PCNTB, PCTN2, SCKL4, Pcnt2, C86676, kendrin, m239Asp, m275Asp, AW476095, DIP2A, AKAP450, CG13459, CG18648, CG33957, CG6735, CP309, d-plp, D-PLP, DmelCG33957, dplp, DPLP, l(3)s2172, PACT, Plp, pPlp

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Organelles

Restrictions: For Research Use only

Images

Western blot analysis of Pericentrin on mouse neonate lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-Pericentrin antibody.

Immunofluorescent staining on NIH-3T3 cells.

Publications

Product: Doxsey, Stein, Evans et al.: "Pericentrin, a highly conserved centrosome protein involved in microtubule organization." in: Cell, Vol. 76, Issue 4, pp. 639-50, 1994 (PubMed).

Young, Dictenberg, Purohit et al.: "Cytoplasmic dynein-mediated assembly of pericentrin and gamma tubulin onto centrosomes." in: Molecular biology of the cell, Vol. 11, Issue 6, pp. 2047-56, 2000 (PubMed).