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Cullin 3 antibody (CUL3) (AA 565-684)

Details for Product anti-CUL3 Antibody No. ABIN968679, Supplier: Log in to see
Antigen
  • CUL-3
  • Cullin-3
  • CUL-3-A
  • Cullin-3-A
  • cul3
  • cul3a
  • CUL-3-B
  • Cullin-3-B
  • cul3b
  • wu:fc37e01
  • CUL3
  • ATCUL3
  • ATCUL3A
  • CUL3A
  • T24P13.25
  • cullin 3
  • cullin 3A
  • PHA2E
  • AI467304
  • AW146203
  • mKIAA0617
Epitope
AA 565-684
16
7
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
90
35
29
2
Host
Mouse
80
9
1
Clonality (Clone)
Monoclonal ()
Conjugate
This Cullin 3 antibody is un-conjugated
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
61
31
25
21
18
11
9
5
5
4
2
1
Supplier
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Immunogen Human CUL-3 aa. 565-684
Clone 3-CUL
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CUL-3 (CUL3 Antibody Abstract)
Background The cullins are a family of proteins that are integral to cell cycle regulation. Members of this family include human Cul proteins, C.elegans Ce-Cul-1, and S. cerevisiae Cdc53. Proper control of cell cycle progression is essential for the prevention of tumorigenesis. Mutant forms of both Ce-Cul-1 and Cdc53 have been implicated in the oncogenic process. The human Cul family of proteins, Cul-1, -2, -3, -4A, -4B, and -5, have also been implicated in oncogenic processes. Abnormal nuclear localization of Cul-2 is seen in a rare hereditary condition known as VHL (von Hippel-Lindau) syndrome. Patients with VHL syndrome possess a mutant VHL gene and develop cancers such as retinal anginomas, CNS hemangioblastomas, and renal cell carcinomas. Cul-3 is widely expressed in normal cells and tissues, and shows increased expression in colon cancer cells. Cul-3 can associate with cyclin E in mammalian cells, and overexpression of Cul-3 targets cyclin E for ubiquination, while deletion of Cul-3 in mice leads to increased cyclin E accumulation and abnormal regulation of S-phase. Thus, the Cul family of proteins may have roles in cell cycle regulation and tumoriogenesis. This antibody is routinely tested by western blot analysis.
Molecular Weight 89 kDa
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-Cullin 3 antibody (CUL3) (AA 565-684) (ABIN968679) Western blot analysis of CUL-3 on a rat cerebrum lysate. Lane 1: 1:10,000, lane 2: 1:...
Immunofluorescence (IF) image for anti-Cullin 3 antibody (CUL3) (AA 565-684) (ABIN968679) Immunofluorescence staining of human endothelial cells.
Western Blotting (WB) image for anti-Cullin 3 antibody (CUL3) (AA 565-684) (ABIN968679) anti-Cullin 3 (CUL3) (AA 565-684) antibody (Image 3)
Product cited in: Hori, Osaka, Chiba et al.: "Covalent modification of all members of human cullin family proteins by NEDD8." in: Oncogene, Vol. 18, Issue 48, pp. 6829-34, 2000 (PubMed).

Singer, Gurian-West, Clurman et al.: "Cullin-3 targets cyclin E for ubiquitination and controls S phase in mammalian cells." in: Genes & development, Vol. 13, Issue 18, pp. 2375-87, 1999 (PubMed).