Myosin Phosphatase, Target Subunit 1 (PPP1R12A) (AA 723-840) antibody

Antigen: Myosin Phosphatase, Target Subunit 1 (PPP1R12A)

Synonyms: MBS, MYPT1, MGC133042, Mypt1, AA792106, AV099298, MGC159084, D10Ertd625e, 1200015F06Rik, 5730577I22Rik, M110, MBSP

Binding Site: AA 723-840

Clonality: Monoclonal (20)

Host: Mouse

Reactivity: Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968768

Additional Information

Alternative name: MYPT1

Immunogen: Rat MYPT1

Cross-Reactivity: Human, Mouse (Murine)

Format: Liquid

Isotype: IgG1

Clone: 20

Description: Protein phosphatase-1 (PP1) is a major serine/threonine phosphatase that is involved in various cellular processes in eukaryotic cells. The PP1 catalytic subunit may be directed to particular subcellular locations by targeting subunits, which can modify the substrate specificity of the enzyme. The PP1 enzyme associated with the myofibrils of striated and smooth muscle forms a complex with Myosin Phosphatase Targeting subunit 1 (MYPT1). MYPT1 contains seven ankyrin repeats in the N-terminal region, and a leucine zipper (LZ) motif in the C-terminus. Alternative splicing of the MYPT1 mRNA leads to expression of a truncated MYPT1 that lacks the leucine zipper region. The expression of MYPT1 splice variants correlate with a smooth muscle phenotype. In the tonic contracting chicken aorta, only the full length MYPT1 is expressed, while in the phasic contracting chicken gizzard, only the truncated MYPT1 is expressed. The leucine zipper motif may be required for association with cGMP-dependent protein kinase I. Thus, MYPT1 may direct not only PP1 enzymatic activity toward myosin, but also may regulate the cGMP responsiveness of PP1 through expression of alternative splice variants.
Synonyms: Myosin Phosphatase Targeting subunit 1

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 130 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of MYPT1 on a rat cerebrum lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-MYPT1 antibody.

Publications

Product: Chen, Chen, Alessi et al.: "Molecular cloning of cDNA encoding the 110 kDa and 21 kDa regulatory subunits of smooth muscle protein phosphatase 1M." in: FEBS letters, Vol. 356, Issue 1, pp. 51-5, 1995 (PubMed).

Kimura, Ito, Amano et al.: "Regulation of myosin phosphatase by Rho and Rho-associated kinase (Rho-kinase)" in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 245-8, 1996 (PubMed).

Khatri, Joyce, Brozovich et al.: "Role of myosin phosphatase isoforms in cGMP-mediated smooth muscle relaxation." in: The Journal of biological chemistry, Vol. 276, Issue 40, pp. 37250-7, 2001 (PubMed).