UHRF1 antibody (AA 199-298)

Catalog No. ABIN968794

This Mouse Monoclonal antibody specifically detects UHRF1 in WB and FM. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for UHRF1 antibody (AA 199-298) (ABIN968794)

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This UHRF1 antibody is un-conjugated

Application: Western Blotting (WB), Fluorescence Microscopy (FM)

Clone: 28-ICBP90

Product Details anti-UHRF1 Antibody

Binding Specificity: AA 199-298

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ICBP90 aa. 199-298

Isotype: IgG2a

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-UHRF1 antibody (ABIN968794)

Hopfner, Mousli, Garnier, Redon, du Manoir, Chatton, Ghyselinck, Oudet, Bronner: "Genomic structure and chromosomal mapping of the gene coding for ICBP90, a protein involved in the regulation of the topoisomerase IIalpha gene expression." in: Gene, Vol. 266, Issue 1-2, pp. 15-23, (2001) (PubMed).

Hopfner, Mousli, Jeltsch, Voulgaris, Lutz, Marin, Bellocq, Oudet, Bronner: "ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression." in: Cancer research, Vol. 60, Issue 1, pp. 121-8, (2000) (PubMed).

Target Details for UHRF1

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Alternative Name: ICBP90

Background: DNA topoisomerases are ubiquitous nuclear enzymes that alter the topology of DNA. Topoisomerase I produces transient single-stranded breaks in DNA, while topoisomerase II produces transient double-stranded breaks in DNA. These DNA breaks are important for DNA replication and chromosome condensation and segregation. Two types of topoisomerase II have been identified, TopoIIalpha and TopoIIbeta. During interphase, TopoIIalpha is found in the nucleoplasm and nucleoli, and in mitosis, TopoIIalpha is found associated with metaphase chromosomes and centromeres. Transcriptional control of TopoIIalpha expression is regulated through multiple gene promoters, which include five inverted CCAAT boxes, one ATF binding sequence, and two GC-rich boxes. ICBP90 was identified in one-hybrid screen of proteins that can bind an inverted CCAAT box. ICBP90 is expressed highest in proliferative tissues such as thymus, liver, and bone marrow. Overexpression of ICBP90 in COS-1-transfected cells leads to enhanced expression of TopoIIalpha. ICBP90 co-localizes with TopoIIalpha in proliferating areas of appendix and in high grade breast carcinomas. Thus, ICBP90 may be an important transcripitonal regulator of TopoIIalpha expression in proliferating cells.

Molecular Weight: 97 kDa