UHRF1 antibody

Catalog No. ABIN2855725

Product Details anti-UHRF1 Antibody

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UHRF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Characteristics: Rabbit Polyclonal antibody to UHRF1 (ubiquitin-like with PHD and ring finger domains 1)
UHRF1 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human UHRF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for UHRF1

Target: UHRF1 (Ubiquitin-Like, Containing PHD and RING Finger Domains, 1 (UHRF1))

Alternative Name: ubiquitin like with PHD and ring finger domains 1 (UHRF1 Products)

Synonyms: ICBP90 antibody, Np95 antibody, RNF106 antibody, hNP95 antibody, hUHRF1 antibody, AL022808 antibody, NP95 antibody, fb97f09 antibody, wu:fb97f09 antibody, zgc:63539 antibody, UHRF1 antibody, ubiquitin like with PHD and ring finger domains 1 antibody, ubiquitin-like, containing PHD and RING finger domains, 1 antibody, ubiquitin-like with PHD and ring finger domains 1 antibody, UHRF1 antibody, Uhrf1 antibody, uhrf1 antibody

Background: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. Multiple transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Nucleus

Molecular Weight: 90 kDa

Gene ID: 29128

UniProt: Q96T88