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Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody

Details for Product No. ABIN968800
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Antigen
Synonyms
P38C-CRK, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, CRK1, Csbp1, Csbp2, Exip, Hog, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, Crk1, p38-alpha, p38MAPK, p38a, csb ... show more
P38C-CRK, CSBP, CSBP1, CSBP2, CSPB1, EXIP, Mxi2, PRKM14, PRKM15, RK, SAPK2A, p38, p38ALPHA, CRK1, Csbp1, Csbp2, Exip, Hog, Prkm14, Prkm15, Sapk2A, p38Hog, p38alpha, Crk1, p38-alpha, p38MAPK, p38a, csbp, mapk14a, mxi2, sapk2, sapk2a, p38b, zp38b, MAPK14, 186F5S, BG:DS00797.3, CG7393, D-p38, D-p38 MAPK, D-p38b, Dm p38b, Dmel\\CG7393, Dmp38b, Dp38, Dp38b, ESTS:186F5S, Mpk34C, anon-sts23, dp38b, p38 MAPK, p38 beta, p38B, p38Kb, p38beta show less
Epitope
pThr180, pTyr182
(90), (75), (19), (17), (16), (13), (13), (12), (10), (9), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(351), (220), (215), (77), (48), (41), (15), (10), (6), (6), (5), (5), (5), (2), (1), (1), (1), (1)
Host
Mouse
(308), (55), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(8), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF)
(293), (148), (90), (64), (54), (50), (23), (19), (12), (11), (9), (3), (2), (2), (1), (1), (1), (1)
Pubmed 3 references available
Quantity 150 µg
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Catalog No. ABIN968800
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Immunogen Human p38 MAPK (pT180/pY182)
Clone OPG-13
Isotype IgG1
Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name p38 MAPK
Background Activation of the immune and inflammatory responses often involves the recognition of bacterial endotoxin (lipopolysaccharide or LPS). Binding of LPS by monocytic cells results in the production and release of proinflammatory cytokines, such as IL-1 and TNF-alpha. LPS-induced signaling cascades involve members of the Ser/Thr protein kinase family known as the mitogen activated protein kinases (MAPKs). MAPK signal transduction pathways mediate the effects of various extracellular stimuli on biological processes such as proliferation, differentiation, and death. The p38 MAP kinases include p38alpha, beta, gamma, and delta. These ser/thr kinases are activated by dual phosphorylation on Thr and Tyr within the motif Thr-Gly-Tyr located in kinase subdomain VIII. Activation of p38 MAPK is mediated specifically by the MAP kinase kinases, MKK3, MKK4, and MKK6. This leads to the activation of multiple transcription factors (NF-kappaB, ATF-2, Elk-1, and CHOP) that induce expression of many different genes, including proinflammatory cytokine genes. Thus, p38 MAPKs are central kinases in muliple signal transduction pathways.
Molecular Weight 38-42 kDa
Research Area Kinases/Phosphatases
Comment

Related Products: ABIN968535, ABIN968632, ABIN967389, ABIN968769

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody Western blot analysis for p38 MAPK (pT180/pY182) (left figure). HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2) were either left untreated (lane 1) or treated with 25 myg/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37°C (lane 2). The top panel was probed with a mouse anti-p38alpha antibody (ABIN968769) and the bottom was probed with the mouse anti-p38 (pT180/pY182) antibody.
anti-Mitogen-Activated Protein Kinase 14 (MAPK14) (pThr180), (pTyr182) antibody (2) Immunofluorescence staining for p38 MAPK (pT180/pY182) (right figure). HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2) were either untreated (upper left and third panels) or were treated for 15 min with 25 µg/mL anisomycin, an antibiotic and protein synthesis inhibitor (upper right and bottom right panels). Cells were fixed in 3.75% paraformaldehyde and permeabilized with 0.2% Triton-X 100. Immunofluorescent staining was performed with either a mouse anti-p38alpha antibody (ABIN968769) (upper left and upper right panels) or with the mouse anti-p38 MAPK (pT180/pY182) antibody (bottom left and bottom right panels).
Product cited in: Han, Lee, Bibbs et al.: "A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells." in: Science (New York, N.Y.), Vol. 265, Issue 5173, pp. 808-11, 1994 (PubMed).

Brunet, Pouysségur: "Identification of MAP kinase domains by redirecting stress signals into growth factor responses." in: Science (New York, N.Y.), Vol. 272, Issue 5268, pp. 1652-5, 1996 (PubMed).

Winston, Chan, Johnson et al.: "Activation of p38mapk, MKK3, and MKK4 by TNF-alpha in mouse bone marrow-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 9, pp. 4491-7, 1997 (PubMed).

Hosts (308), (55), (2), (1)
Reactivities (351), (220), (215), (77), (48), (41), (15), (10), (6), (6), (5), (5), (5), (2), (1), (1), (1), (1)
Applications (293), (148), (90), (64), (54), (50), (23), (19), (12), (11), (9), (3), (2), (2), (1), (1), (1), (1)
Conjugates (8), (8), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (90), (75), (19), (17), (16), (13), (13), (12), (10), (9), (6), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN968800
Contact our Customer Service for availability and price in your country.
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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