Nicastrin (NCSTN) (AA 168-289) antibody

Antigen: Nicastrin (NCSTN)

Synonyms: APH2, KIAA0253, RP11-517F10.1, Nct, Nic, S5A, agro, dNct, DmelCG7012, CG7012, Aph2, AA727311, D1Dau13e, Kiaa0253, mKIAA0253, 9430068N19Rik, NCSTN, MGC126920, DKFZp469O2418

Binding Site: AA 168-289

Clonality: Monoclonal (35)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968805

Additional Information

Alternative name: Nicastrin

Immunogen: Human Nicastrin

Cross-Reactivity: Chicken, Dog (Canine), Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG2a

Clone: 35

Description: Amyloid precursor protein (APP) gene encodes multiple APPs ranging from 695 to 770 amino acids. The unprocessed form of APP is a putative cell surface receptor that possesses neurite-promoting activity, and is involved in synaptic vesicle recycling. Processing of APP by sequential enzymatic activity of beta- and gamma-secretase, and the presenilin proteins PS1 and PS2, produces APP fragments that may have unique functions. beta-secretase activity cleaves the extracellular portion of APP leading to asecreted APP form, while gamma-secretase activity produces beta-Amyloid peptide (39-43 amino acids). The Abeta peptide produces abnormal plaques in the cerebral cortex and blood vesselwalls during Alzheimer's disease. Nicastrin is a PS1 associated transmembrane protein,that contains N-terminal glycosylation and myristoylation sites. Nicastrin can bind full length APP and the fragments produced by gamma-secretase. In C. elegans, suppression of nicastrin expression produces phenotypes that mimic those produced when notch signaling proteins are suppressed. In Drosophila, deficiencies in nicastrin prevent cleavage of the intracellular portion of Notch. Thus, nicastrin may be a functional component of presenilins and gamma-secretase complexes, which process Notch and APP transmembrane receptors.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 110 kDa

Comments:

Related Products: ABIN967389, ABIN968553

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Research Area: Stem Cells, Alzheimer's Disease, Immunology

Restrictions: For Research Use only

Images

Western blot analysis of Nicastrin on WI-38 lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of Nicastrin.

Publications

Product: Yu, Nishimura, Arawaka et al.: "Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing." in: Nature, Vol. 407, Issue 6800, pp. 48-54, 2000 (PubMed).

Chen, Yu, Arawaka et al.: "Nicastrin binds to membrane-tethered Notch." in: Nature cell biology, Vol. 3, Issue 8, pp. 751-4, 2001 (PubMed).