HNF1B antibody (AA 1-101)

Catalog No. ABIN968855

The Mouse Monoclonal anti-HNF1B antibody has been validated for WB and IF. It is suitable to detect HNF1B in samples from Human, Rat, Mouse and Dog. There are 3+ publications available.

Quick Overview for HNF1B antibody (AA 1-101) (ABIN968855)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Human, Rat, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 25-HNF

Product Details anti-HNF1B Antibody

Binding Specificity: AA 1-101

Cross-Reactivity: Rat (Rattus), Human, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse HNF-1beta aa. 1-101

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968547, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-HNF1B antibody (ABIN968855)

Soubt, Marksitzer, Menoud, Nagamine: "Role of tissue-specific transcription factor LFB3 in a cyclic AMP-responsive enhancer of the urokinase-type plasminogen activator gene in LLC-PK1 cells." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4698-706, (1998) (PubMed).

Ringeisen, Rey-Campos, Yaniv: "The transactivation potential of variant hepatocyte nuclear factor 1 is modified by alternative splicing." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25706-11, (1994) (PubMed).

De Simone, De Magistris, Lazzaro, Gerstner, Monaci, Nicosia, Cortese: "LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia." in: The EMBO journal, Vol. 10, Issue 6, pp. 1435-43, (1991) (PubMed).

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: HNF-1beta

Background: HNF's (Hepatocyte Nuclear Factor) are POU-homeodomain-containing liver enriched transcription factors that include HNF1-alpha, HNF1-beta, HNF-3alpha, HNF-3beta, HNF-4 and HNF-6. HNF-1 is expressed in liver, kidney, and intestine, while HNF-1beta is expressed highest in kidney with lower levels in intestine, lung, and liver. Both HNF-1 and HNF-1beta have short N-terminal alpha-helical segments that facilitate homodimerization (A-domain), a region related to POU-specific A-box (B-domain), and a homeodomain (C-domain). The HNF-1 and HNF-1beta homo- and hetero-dimers transactivate genes required for development of various tissues. Both transcription factors can bind the consensus HNF1 inverted palindromic sequence GTTAATNATTAAC. HNF-1 represses its own transcription and data suggests that there is coordinate regulation between HNF-1 and HNF-4. Although HNF-4 induces HNF-1 transcription, HNF-1 downregulates HNF-4 activity by binding to the AF2 region in the activation domain of HNF-4. Thus, HNF transcription factors may be involved in various aspects of the regulation of gene transcription throughout development.
Synonyms: Hepatocyte Nuclear Factor-1beta

Molecular Weight: 51 kDa

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation