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HNF1 Homeobox B (HNF1B) (AA 1-101) antibody

Details for Product No. ABIN968855, Supplier: Login to see
Antigen
  • TCF2
  • HNF1B
  • FJHN
  • HNF-1B
  • HNF1beta
  • HNF2
  • HPC11
  • LF-B3
  • LFB3
  • MODY5
  • TCF-2
  • VHNF1
  • hnf-1beta
  • hnf1-beta
  • hnf1beta
  • lfb3
  • tcf2
  • vhnf1
  • vHNF-1
  • AI385728
  • AI987804
  • HNF-1Beta
  • Hnf1beta
  • Tcf-2
  • Tcf2
  • vHNF1
  • HNF-1b
  • chunp6877
  • hnf1b
  • mst
Epitope
AA 1-101
23
18
16
13
10
9
5
4
3
3
2
2
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
113
40
40
8
8
8
6
3
3
2
1
1
1
Host
Mouse
84
18
16
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
98
56
11
10
7
6
2
2
1
Supplier
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Immunogen Mouse HNF-1beta aa. 1-101
Clone 25-HNF
Isotype IgG1
Cross-Reactivity Rat (Rattus), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name HNF-1beta (HNF1B Antibody Abstract)
Background HNF's (Hepatocyte Nuclear Factor) are POU-homeodomain-containing liver enriched transcription factors that include HNF1-alpha, HNF1-beta, HNF-3alpha, HNF-3beta, HNF-4 and HNF-6. HNF-1 is expressed in liver, kidney, and intestine, while HNF-1beta is expressed highest in kidney with lower levels in intestine, lung, and liver. Both HNF-1 and HNF-1beta have short N-terminal alpha-helical segments that facilitate homodimerization (A-domain), a region related to POU-specific A-box (B-domain), and a homeodomain (C-domain). The HNF-1 and HNF-1beta homo- and hetero-dimers transactivate genes required for development of various tissues. Both transcription factors can bind the consensus HNF1 inverted palindromic sequence GTTAATNATTAAC. HNF-1 represses its own transcription and data suggests that there is coordinate regulation between HNF-1 and HNF-4. Although HNF-4 induces HNF-1 transcription, HNF-1 downregulates HNF-4 activity by binding to the AF2 region in the activation domain of HNF-4. Thus, HNF transcription factors may be involved in various aspects of the regulation of gene transcription throughout development.
Synonyms: Hepatocyte Nuclear Factor-1beta
Molecular Weight 51 kDa
Pathways
Comment

Related Products: ABIN968547, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.
Supplier Images
Western Blotting (WB) image for anti-HNF1 Homeobox B (HNF1B) (AA 1-101) antibody (ABIN968855) Western blot analysis of HNF-1beta on a rat kidney lysate. Lane 1: 1:250, lane 2: 1:5...
Immunofluorescence (IF) image for anti-HNF1 Homeobox B (HNF1B) (AA 1-101) antibody (ABIN968855) Immunofluorescence staining of normal rat kidney.
Western Blotting (WB) image for anti-HNF1 Homeobox B (HNF1B) (AA 1-101) antibody (ABIN968855) anti-HNF1 Homeobox B (HNF1B) (AA 1-101) antibody (Image 3)
Product cited in: Soubt, Marksitzer, Menoud et al.: "Role of tissue-specific transcription factor LFB3 in a cyclic AMP-responsive enhancer of the urokinase-type plasminogen activator gene in LLC-PK1 cells." in: Molecular and cellular biology, Vol. 18, Issue 8, pp. 4698-706, 1998 (PubMed).

Ringeisen, Rey-Campos, Yaniv: "The transactivation potential of variant hepatocyte nuclear factor 1 is modified by alternative splicing." in: The Journal of biological chemistry, Vol. 268, Issue 34, pp. 25706-11, 1994 (PubMed).

De Simone, De Magistris, Lazzaro et al.: "LFB3, a heterodimer-forming homeoprotein of the LFB1 family, is expressed in specialized epithelia." in: The EMBO journal, Vol. 10, Issue 6, pp. 1435-43, 1991 (PubMed).