Mitogen-Activated Protein Kinase 8 (MAPK8) (pThr183,pTyr185) antibody

Antigen: Mitogen-Activated Protein Kinase 8 (MAPK8)

Synonyms: JNK, JNK1, PRKM8, SAPK1, JNK1A2, JNK21B1/2, Prkm8, AI849689, Bsk, D-JNK, D-junk, DBSK/JNK, DJNK, DJNK/bsk, JNK/SAPK, Junk, SAPKa, dJUN, DmelCG5680, CG5680, T10F20.15

Binding Site: pThr183,pTyr185

Clonality: Monoclonal (41)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN968867

Additional Information

Alternative name: JNK

Immunogen: Phosphorylated Human JNK/SAPK (pT183/pY185) Peptide

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 41

Description: The Ras signaling pathway links the signals from growth factor receptors with the activation of the MAPK kinase cascade of phosphorylation leading to cell growth and differentiation. External stimuli, like endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression, ultimately dictating an adaptation to the new environment. Small GTPases of the Rho family, including cdc42, Rac1, and Rho, transmit the stress signals that initiate the signal cascade. JNK is a c-Jun kinase that was also identified as SAPK1 and MAPKp49. JNK/SAPK, along with p38 and RK5/BMK1, comprise three classes of stress-activated MAPK groups. Complete activation of JNK/SAPK requires the phosphorylation of both Thr183 and Tyr185, which are located in a Thr-X-Tyr motif. The activation of these residues is believed to be carried out by MKK4 and MKK7. Active JNK/SAPK phosphorylates other kinases and multiple transcription factors that induce expression of genes, such as proinflammatory cytokines.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 43 & 56 kDa

Comments:

Related Products: ABIN968535, ABIN968632, ABIN967389, ABIN968074

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis for JNK/SAPK (pT183/pY185). HeLa cells (Human cervical epitheloid carcinoma, ATCC CCL-2) were either left untreated (lane 1) or treated with 25 µg/mL anisomycin, an antibiotic and protein synthesis inhibitor, for 15 min at 37°C (lane 2). The top panel was probed with a mouse anti-JNK/SAPK1 antibody (ABIN968074) and the bottom panel was probed with the mouse anti-JNK/SAPK1 (pT183/pY185) antibody at a 1:250 dilution with bands observable at ~ 43 kDa & ~ 56 kDa.

Publications

Product: Hinton, Henderson, Blanks et al.: "Monoclonal antibodies react with neuronal subpopulations in the human nervous system." in: The Journal of comparative neurology, Vol. 267, Issue 3, pp. 398-408, 1988 (PubMed).

Fleming, Armstrong, Morrice et al.: "Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7." in: The Biochemical journal, Vol. 352 Pt 1, Issue 3, pp. 145-54, 2001 (PubMed).

Muyembe, Maes, Makulu et al.: "The hem of the garment." in: Seminars in dialysis, Vol. 14, Issue 1, pp. 76, 2001 (PubMed).

Kyriakis, Avruch: "Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation." in: Physiological reviews, Vol. 81, Issue 2, pp. 807-69, 2001 (PubMed).