JNK antibody (pThr183)

Catalog No. ABIN968867

The Mouse Monoclonal anti-JNK antibody has been validated for WB and FACS. It is suitable to detect JNK in samples from Human, Mouse and Rat. There are 5+ publications available.

Quick Overview for JNK antibody (pThr183) (ABIN968867)

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This JNK antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Clone: 41-JNK-SAPK

Product Details anti-JNK Antibody

Binding Specificity: pThr183

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Phosphorylated Human JNK/SAPK (pT183/pY185) Peptide

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968535, ABIN968632, ABIN967389, ABIN968074

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-JNK antibody (ABIN968867)

Fleming, Armstrong, Morrice, Paterson, Goedert, Cohen: "Synergistic activation of stress-activated protein kinase 1/c-Jun N-terminal kinase (SAPK1/JNK) isoforms by mitogen-activated protein kinase kinase 4 (MKK4) and MKK7." in: The Biochemical journal, Vol. 352 Pt 1, Issue 3, pp. 145-54, (2001) (PubMed).

Kyriakis, Avruch: "Mammalian mitogen-activated protein kinase signal transduction pathways activated by stress and inflammation." in: Physiological reviews, Vol. 81, Issue 2, pp. 807-69, (2001) (PubMed).

Muyembe, Maes, Makulu, Ghysels, Vandeven, Vandepitte: "The hem of the garment." in: Seminars in dialysis, Vol. 14, Issue 1, pp. 76, (2001) (PubMed).

Mohit, Martin, Miller: "p493F12 kinase: a novel MAP kinase expressed in a subset of neurons in the human nervous system." in: Neuron, Vol. 14, Issue 1, pp. 67-78, (1995) (PubMed).

Hinton, Henderson, Blanks, Rudnicka, Miller: "Monoclonal antibodies react with neuronal subpopulations in the human nervous system." in: The Journal of comparative neurology, Vol. 267, Issue 3, pp. 398-408, (1988) (PubMed).

Target Details for JNK

Target: JNK (MAPK8) (Mitogen-Activated Protein Kinase 8 (MAPK8))

Alternative Name: JNK

Background: The Ras signaling pathway links the signals from growth factor receptors with the activation of the MAPK kinase cascade of phosphorylation leading to cell growth and differentiation. External stimuli, like endotoxins, UV irradiation, heat, and hyperosmolarity, induce an array of cellular responses that culminate with gene expression, ultimately dictating an adaptation to the new environment. Small GTPases of the Rho family, including cdc42, Rac1, and Rho, transmit the stress signals that initiate the signal cascade. JNK is a c-Jun kinase that was also identified as SAPK1 and MAPKp49. JNK/SAPK, along with p38 and RK5/BMK1, comprise three classes of stress-activated MAPK groups. Complete activation of JNK/SAPK requires the phosphorylation of both Thr183 and Tyr185, which are located in a Thr-X-Tyr motif. The activation of these residues is believed to be carried out by MKK4 and MKK7. Active JNK/SAPK phosphorylates other kinases and multiple transcription factors that induce expression of genes, such as proinflammatory cytokines.

Molecular Weight: 43 & 56 kDa

Pathways: MAPK Signaling, WNT Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, Signaling of Hepatocyte Growth Factor Receptor, S100 Proteins