Images for product: anti-PKA-R2 beta (pSer114) antibody
Westen Blot: Rat Cerebrum lysate was either left untreated (lane 1) or treated (lane 2) with 150U/ml of lambda phosphatase for 1 hour at 37°C. The top panel was probed with PKA RIIbeta (ABIN968072). The bottom panel was probed with anti-PKA RIIbeta (pS114). Recommended to use antibody in range of 1:1000 dilution.
Immunofluorescent staining of SK-N-SH cells. Cells were seeded in a 384 well collagen coated Microplates at ~ 8,000 cells per well. After overnight incubation, cells were stained using the methanol fix/perm protocol and the anti- PKARIIb p S114 antibody. The second step reagent was Alexa Fluor® 488 goat anti mouse Ig (Invitrogen). The image was taken on a Pathway 855 or 435 imager using a 20x objective. This antibody also stained SH-SY5Y, and C6 cells using both the Triton X100 and methanol fix/perm protocols.