Images for product: anti-Gephyrin (GPHN) (AA 569-726) antibody
Western blot analysis of Gephyrin. Lysates from rat cerebrum (5 µg/lane), S2 cells (7.5 µg/lane), and 0-12 hours Drosophila embryos (7.5 µg/lane) were probed with anti- Gephyrin at concentrations of 4 µg/ml (lane 1), 2 µg/ml (lane 2), and 1 µg/ml (lane 3). Anti-Gephyrin detects a band of 93 kDa in rat cerebrum and 74 kDa in S2 cells and Drosophila embryos.
Gephyrin immunochemical staining of 0-12 hour embryos. Formalin-fixed embryos kept in ethanol at -20°C, were re-hydrated and incubated with anti-Gephyrin at 1 µgg/ml in TBST. After extensive washes to remove unbound antibody, Gephyrin signal was visualized with ALEXA fluor 488 secondary antibody (1/300) and confocal microscopy. Gephyrin staining is found throughout the cellularizing embryo, and it appears more intense in the nuclei with nuclear dots stained.