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CHD3 antibody (Chromodomain Helicase DNA Binding Protein 3)

Details for Product anti-CHD3 Antibody No. ABIN969052, Supplier: Log in to see
Antigen
  • 2600010P09Rik
  • AF020312
  • CG9594
  • CHD-3
  • chd3
  • CHD3
  • Chd7
  • dCHD3
  • DmCHD3
  • Dmel\\CG9594
  • LOC568230
  • mi-2a
  • Mi-2a
  • Mi2-ALPHA
  • mi2-alpha
  • Prp7
  • Prp9-1
  • zfh
  • ZFH
Reactivity
Human, Mouse (Murine)
66
30
17
2
1
1
Host
Mouse
40
32
2
Clonality (Clone)
Monoclonal ()
Conjugate
This CHD3 antibody is un-conjugated
4
4
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
48
31
23
18
15
13
12
11
6
3
2
1
Supplier
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Available images

Immunogen Purified recombinant fragment of human CHD3 expressed in E. coli.
Clone 2G4
Isotype IgG1
Purification Ascites
Alternative Name CHD3 (CHD3 Antibody Abstract)
Background This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Molecular Weight 226 kDa
Gene ID 1107
HGNC 1107
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, ICC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Flow Cytometry (FACS) image for anti-CHD3 antibody (Chromodomain Helicase DNA Binding Protein 3) (ABIN969052) Figure 1: Flow cytometric analysis of K562 cells using CHD3 mouse mAb (green) and neg...
Immunocytochemistry (ICC) image for anti-CHD3 antibody (Chromodomain Helicase DNA Binding Protein 3) (ABIN969052) Figure 2: Immunofluorescence analysis of Hela cells using CHD3 mouse mAb (green) . Bl...
Immunohistochemistry (IHC) image for anti-CHD3 antibody (Chromodomain Helicase DNA Binding Protein 3) (ABIN969052) Figure 3: Immunohistochemical analysis of paraffin-embedded colon cancer tissues usin...
Western Blotting (WB) image for anti-CHD3 antibody (Chromodomain Helicase DNA Binding Protein 3) (ABIN969052) Figure 4: Western blot analysis using CHD3 mouse mAb against Hela (1), K562 (2), Jurk...
Product cited in: Burd, Kinyamu, Miller, Archer: "UV radiation regulates Mi-2 through protein translation and stability." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34976-82, 2008 (PubMed).