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KSHV ORF45 antibody

Details for Product No. ABIN969254, Supplier: Log in to see
Antigen
Reactivity
Kaposi's Sarcoma-associated Herpesvirus (KSHV)
3
Host
Mouse
2
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
3
3
1
1
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Immunogen Purified recombinant fragment of KSHV ORF45 expressed in E. coli.
Clone 2D4A5
Isotype IgG1
Purification Ascites
Background Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi's sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman's disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions
Gene ID 4961474
HGNC 4961474
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Immunohistochemistry (IHC) image for anti-KSHV ORF45 antibody (ABIN969254) Figure 1: Immunocytochemistry analysis of TPA induced BCBL-1 cells (A) and uninduced ...
Western Blotting (WB) image for anti-KSHV ORF45 antibody (ABIN969254) Figure 2: Western blot analysis using KSHV ORF45 mouse mAb against KSHV ORF45 recombi...
Product cited in: Zhu, Eisenach: "Cyclooxygenase-1 in the spinal cord is altered after peripheral nerve injury." in: Anesthesiology, Vol. 99, Issue 5, pp. 1175-9, 2003 (PubMed).

Zhu, Yuan: "The ORF45 protein of Kaposi's sarcoma-associated herpesvirus is associated with purified virions." in: Journal of virology, Vol. 77, Issue 7, pp. 4221-30, 2003 (PubMed).

Zhu, King, Smith et al.: "A Kaposi's sarcoma-associated herpesviral protein inhibits virus-mediated induction of type I interferon by blocking IRF-7 phosphorylation and nuclear accumulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 8, pp. 5573-8, 2002 (PubMed).

Background publications Chang, Cesarman, Pessin et al.: "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma." in: Science (New York, N.Y.), Vol. 266, Issue 5192, pp. 1865-9, 1995 (PubMed).