KSHV ORF45 antibody

Antigen: KSHV ORF45

Clonality: Monoclonal (2D4A5)

Host: Mouse

Reactivity: Kaposi's Sarcoma-associated Herpesvirus (KSHV)

Application: ELISA, Immunofluorescence (IF), Western Blotting (WB)

Catalog no.: ABIN969254

Additional Information

Gene ID: 4961474

Immunogen: Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 2D4A5

Description: Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions
Synonyms: ORF45

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Restrictions: For Research Use only

Publications

Product: Chang, Cesarman, Pessin et al.: "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma." in: Science (New York, N.Y.), Vol. 266, Issue 5192, pp. 1865-9, 1995 (PubMed).

Zhu, King, Smith et al.: "A Kaposi's sarcoma-associated herpesviral protein inhibits virus-mediated induction of type I interferon by blocking IRF-7 phosphorylation and nuclear accumulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 8, pp. 5573-8, 2002 (PubMed).

Zhu, Yuan: "The ORF45 protein of Kaposi's sarcoma-associated herpesvirus is associated with purified virions." in: Journal of virology, Vol. 77, Issue 7, pp. 4221-30, 2003 (PubMed).

Zhu, Eisenach: "Cyclooxygenase-1 in the spinal cord is altered after peripheral nerve injury." in: Anesthesiology, Vol. 99, Issue 5, pp. 1175-9, 2003 (PubMed).