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KSHV ORF45 antibody
Kaposi's Sarcoma-associated Herpesvirus (KSHV)
Alternatives ELISA, Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
|Price||407.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.|
Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions
Western Blotting: 1/500 - 1/2000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Buffer||Ascitic fluid containing 0.03% sodium azide.|
|Preservative||0.03% sodium azide|
|Storage||Store at 4℃, for long term storage, store at -20℃.|
|Restrictions||For Research Use only|
Chang, Cesarman, Pessin et al.: "Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi's sarcoma." in: Science (New York, N.Y.), Vol. 266, Issue 5192, pp. 1865-9, 1995 (PubMed).
Zhu, King, Smith et al.: "A Kaposi's sarcoma-associated herpesviral protein inhibits virus-mediated induction of type I interferon by blocking IRF-7 phosphorylation and nuclear accumulation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 8, pp. 5573-8, 2002 (PubMed).
Zhu, Yuan: "The ORF45 protein of Kaposi's sarcoma-associated herpesvirus is associated with purified virions." in: Journal of virology, Vol. 77, Issue 7, pp. 4221-30, 2003 (PubMed).
Zhu, Eisenach: "Cyclooxygenase-1 in the spinal cord is altered after peripheral nerve injury." in: Anesthesiology, Vol. 99, Issue 5, pp. 1175-9, 2003 (PubMed).
|Reactivities||Kaposi's Sarcoma-associated Herpesvirus (KSHV) (1)|
|Applications||ELISA (3), Western Blotting (WB) (3), Immunocytochemistry (ICC) (2)|