LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibody

Details for Product No. ABIN969354, Supplier: Log in to see
Antigen
  • PINCH
  • PINCH-1
  • PINCH1
  • Pinch1
  • RGD1560732
  • LIMS3
  • LIMS3L
  • 2310016J22Rik
  • 4921524A02Rik
  • AI507642
  • AU021743
  • AW551584
  • C430041B13Rik
  • Lims1l
  • LIM and senescent cell antigen-like domains 1
  • LIMS1
  • Lims1
Reactivity
Human
53
32
32
5
4
4
4
4
4
3
3
2
2
2
Host
Mouse
43
11
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
38
18
14
13
8
8
6
4
3
1
1
1
Options
Supplier
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Supplier Product No.
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Immunogen Purified recombinant fragment of human PINCH expressed in E. Coli.
Clone 5G7
Isotype IgG1
Purification Ascites
Alternative Name PINCH (LIMS1 Antibody Abstract)
Background The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 37 kDa
Gene ID 3987
HGNC 3987
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, ICC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage
Supplier Images
ELISA image for anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibody (ABIN969354) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibody (ABIN969354) Figure 2: Flow cytometric analysis of Hela cells using PINCH mouse mAb (blue) and neg...
Immunocytochemistry (ICC) image for anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibody (ABIN969354) Figure 3: Immunofluorescence analysis of HepG2 cells using PINCH mouse mAb (green) . ...
Western Blotting (WB) image for anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibody (ABIN969354) Figure 4: Western blot analysis using PINCH mouse mAb against A549 (1), Jurkat (2), a...
Western Blotting (WB) image for anti-LIM and Senescent Cell Antigen-Like Domains 1 (LIMS1) antibody (ABIN969354) Figure 5: Western blot analysis using PINCH mAb against human PINCH (AA: 87-249) reco...
Product cited in: Yang, Wang, Hawkins, Chen, Vaynberg, Mao, Tu, Zuo, Wang, Wang, Wu, Tjandra, Qin: "Structural basis of focal adhesion localization of LIM-only adaptor PINCH by integrin-linked kinase." in: The Journal of biological chemistry, Vol. 284, Issue 9, pp. 5836-44, 2009 (PubMed).

Chiswell, Zhang, Murphy, Boggon, Calderwood: "The structural basis of integrin-linked kinase-PINCH interactions." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 52, pp. 20677-82, 2008 (PubMed).

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