SMAD, Mothers Against DPP Homolog 6 (SMAD6) antibody

Antigen: SMAD, Mothers Against DPP Homolog 6 (SMAD6)

Synonyms: MADH6, MADH7, HsT17432, madh6, madh7, Xsmad6, hst17432

Clonality: Monoclonal (5H3)

Host: Mouse

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN969405

Additional Information

Alternative name: SMAD6

Gene ID: 4091

Immunogen: Purified recombinant fragment of human SMAD6 expressed in E. Coli.

Format: Liquid

Isotype: IgG1

Clone: 5H3

Description: Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members. has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD. Binds to regulatory elements in target promoter regions.Tissue specificity: Ubiquitous in various organs, with higher levels in lung. Isoform B is up-regulated in diseased heart tissue.
Synonyms: MADH6, MADH7, HsT17432, SMAD6

Molecular Weight: 53kDa

Application Details

Application Notes: Western Blotting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascites

Buffer: Ascitic fluid containing 0.03% sodium azide.

Preservative: 0.03% sodium azide

Storage: Store at 4℃, for long term storage, store at -20℃.

Research Area: Cardiovascular, Hypertrophy, Cardiogenesis, Signaling, Transcription Factors

Restrictions: For Research Use only

Publications

Product: Shen, Chen, Wang et al.: "Smad6 interacts with Runx2 and mediates Smad ubiquitin regulatory factor 1-induced Runx2 degradation." in: The Journal of biological chemistry, Vol. 281, Issue 6, pp. 3569-76, 2006 (PubMed).