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Checkpoint Kinase 1 (CHEK1) antibody

Antigen

Checkpoint Kinase 1 (CHEK1)
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1
Clonality Monoclonal (2G1D5)
Host
Alternatives

Mouse
Reactivity
Alternatives

Human, Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives ELISA, Western Blotting (WB)
2 references available
Catalog no. ABIN969476
Quantity 0.1ml  (Variants)
Price 286.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name CHK1
Gene ID 1111
Immunogen Purified recombinant fragment of CHK1 expressed in E. Coli.
Format Liquid
Isotype IgG1
Clone 2G1D5
Description CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Synonyms: CHEK1
Molecular Weight 56kDa

Application Details
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Application Notes Western Blotting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Purification Purified
Buffer PBS containing 0.03% sodium azide.
Preservative 0.03% sodium azide
Storage Store at 4℃, for long term storage, store at -20℃.
Research Area Kinases/Phosphatases, Cell Cycle
Restrictions For Research Use only

Publications
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Product OConnell, Raleigh, Verkade et al.: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts Rabbit (226), Mouse (19), Goat (6), Sheep (4)
Reactivities Human (243), Rat (Rattus) (131), Mouse (Murine) (123), Horse (Equine) (36), Cow (Bovine) (22), Chicken (21), Dog (Canine) (20), Pig (Porcine) (19), Monkey (4), Xenopus laevis (2), Yeast (2), Zebrafish (2), Cat (Feline) (1), Rabbit (1), Yeast (Saccharomyces cerevisiae) (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (149), ELISA (92), Immunofluorescence (IF) (91), Immunohistochemistry (IHC) (46), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunoprecipitation (IP) (18), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (15), Immunocytochemistry (ICC) (10), Immunoelectron Microscopy (IEM) (5), Dot Blot (DB) (2), Dot Blot (Dot) (2), Flow Cytometry (FACS) (2)
Conjugates Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), Biotin (5), Cy3 (5), Cy5 (5), Cy5.5 (5), Cy7 (5), FITC (5), Gold (5), HRP (5), PE (5), PE,Cy3 (4), PE,Cy5 (4), PE,Cy5.5 (4), PE,Cy7 (4), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)
Epitopes pSer317 (37), pSer280 (34), pSer345 (30), Ser296 (18), Internal Region (7), C-Term (6), pSer296 (6), Ser280 (4), AA 312-327 (3), N-Term (3), pSer296,Internal Region (3), Center (2), AA 1-474 (1), AA 250-300 (1), AA 259-311 (1), Internal Region,AA 251-279 (1), Middle Region (1), pSer286 (1), pSer301 (1)