Checkpoint Kinase 1 (CHEK1) antibody

Details for Product No. ABIN969476
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Antigen
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, chk1, id:ibd2720, zgc:56093, An08g10320, AO090003000441
Reactivity
Human, Mouse (Murine)
(345), (124), (123), (36), (26), (25), (14), (4), (3), (2), (2), (2), (1)
Host
Mouse
(308), (34), (9), (5)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Application
ELISA, Western Blotting (WB)
(264), (137), (76), (57), (50), (36), (18), (17), (6), (1), (1), (1)
Pubmed 2 references available
Catalog no. ABIN969476
Quantity 100 µL
Price
286.00 $   Plus shipping costs $45.00
Options
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  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Purified recombinant fragment of CHK1 expressed in E. coli.
Clone 2G1D5
Isotype IgG1
Purification Purified
Alternative Name CHK1
Background Synonyms:
CHEK1
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Molecular Weight 56 kDa
Gene ID 1111
Research Area Kinases/Phosphatases, Cell Cycle
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
anti-Checkpoint Kinase 1 (CHEK1) antibody Figure 1: Western blot analysis using CHK1 mouse mAb against A431 (1), Hela (2), NIH/3T3 (3) and K562 (4) cell lysate.
anti-Checkpoint Kinase 1 (CHEK1) antibody (2) Figure 2: Western blot analysis using CHK1 mouse mAb against HEK293T cells transfected with the pCMV6-ENTRY control (1) and pCMV6-ENTRY CHK1 cDNA (2) .
Product cited in: OConnell, Raleigh, Verkade et al.: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Hosts (308), (34), (9), (5)
Reactivities (345), (124), (123), (36), (26), (25), (14), (4), (3), (2), (2), (2), (1)
Applications (264), (137), (76), (57), (50), (36), (18), (17), (6), (1), (1), (1)
Conjugates (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (45), (41), (31), (29), (13), (8), (8), (7), (4), (3), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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