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Details for Product No. ABIN969476

Checkpoint Kinase 1 (CHEK1) antibody

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Antigen
Synonyms CHK1, Chk1, rad27, C85740, CHEK1, chk1, id:ibd2720, zgc:56093, An08g10320, AO090003000441
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Western Blotting (WB)
Pubmed 2 references available
Catalog no. ABIN969476
Quantity 100 µL
Price
286.00 $   Plus shipping costs $45.00
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Availability Will be delivered in 3 to 4 Business Days
Immunogen Purified recombinant fragment of CHK1 expressed in E. coli.
Clone 2G1D5
Isotype IgG1
Purification Purified
Alternative Name CHK1
Background Synonyms:
CHEK1
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Molecular Weight 56 kDa
Gene ID 1111
Research Area Kinases/Phosphatases, Cell Cycle
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Product cited in: OConnell, Raleigh, Verkade et al.: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Alternatives for antigen "Checkpoint Kinase 1 (CHEK1)", type "Antibodies"
Hosts (291), (36), (11), (5)
Reactivities (332), (126), (124), (36), (26), (25), (14), (4), (3), (3), (2), (1), (1)
Applications (247), (135), (66), (66), (50), (37), (21), (19), (7), (3), (3), (2), (1)
Conjugates (5), (5), (5), (5), (5), (5), (5), (5), (5), (5), (5)
Epitopes (39), (39), (31), (26), (13), (7), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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