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Checkpoint Kinase 1 (CHEK1) antibody
|Synonyms||CHK1, Chk1, rad27, C85740, CHEK1, LOC398191, chk1, chek1|
Human, Mouse (Murine)
Alternatives ELISA, Western Blotting (WB)
|2 references available|
|Price||286.00 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 3 to 4 Business Days|
|Immunogen||Purified recombinant fragment of CHK1 expressed in E. Coli.|
CHK1, also known as CHEK1, it is a kinase that phosphorylates cdc25, an important phosphatase in cell cycle control, particularly for entry into mitosis. Cdc25, when phosphorylated on serine 216 by chk1 becomes bound by an adaptor protein in the cytoplasm. Therefore it is inhibited from removing the inhibiting phosphate from MPF (mitotic/maturation promoting factor) added by Wee1. Consequently, a cell is prevented from entering mitosis. Chk1 can also phosphorylate p53 at Ser20 in vitro.
Western Blotting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Buffer||PBS containing 0.03% sodium azide.|
|Preservative||0.03% sodium azide|
|Storage||Store at 4℃, for long term storage, store at -20℃.|
|Research Area||Kinases/Phosphatases, Cell Cycle|
|Restrictions||For Research Use only|
OConnell, Raleigh, Verkade et al.: "Chk1 is a wee1 kinase in the G2 DNA damage checkpoint inhibiting cdc2 by Y15 phosphorylation." in: The EMBO journal, Vol. 16, Issue 3, pp. 545-54, 1997 (PubMed).
Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).