Mouse anti-Human IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN969481, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
5027
2578
1931
1069
1007
857
330
299
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Human
2911
2859
2405
1752
1485
796
623
442
381
380
355
340
307
293
208
148
139
114
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Mouse
6893
4912
1878
894
703
401
92
54
52
21
9
8
5
5
2
1
1
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Human IgG was isolated from human sera and purified by chromatography.
Clone 4D2D9G8-4A10
Isotype IgG1
Characteristics Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Purification Purified
Background Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Molecular Weight 50 kDa
Research Area Immunology, Secondary Antibodies
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Heavy & Light Chain) antibody (ABIN969481) Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb...
Product cited in: Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).