Mouse anti-Human IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN969481, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4679
2536
1881
1066
1006
860
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Human
2848
2739
2297
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Mouse
6708
4832
1795
890
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality (Clone)
Monoclonal ()
Application
ELISA, Western Blotting (WB)
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Immunogen Human IgG was isolated from human sera and purified by chromatography.
Clone 4D2D9G8-4A10
Isotype IgG1
Characteristics Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Purification Purified
Background Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
Molecular Weight 50 kDa
Research Area Immunology, Secondary Antibodies
Application Notes Recommended Dilution:
ELISA: 1/10000, WB: 1/500 - 1/2000
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Storage 4 °C/-20 °C
Storage Comment Store at 4 °C or at -20 °C for long term.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Heavy & Light Chain) antibody (ABIN969481) Figure 1: Western blot analysis using IgG mouse mAb (lane 1, 2) and Albumin mouse mAb...
Product cited in: Klonisch, Panayotou, Edwards, Jackson, Berger, Delves, Lund, Roitt: "Enhancement in antigen binding by a combination of synergy and antibody capture." in: Immunology, Vol. 89, Issue 2, pp. 165-71, 1996 (PubMed).