NFKBIA antibody
Quick Overview for NFKBIA antibody (ABIN121497)
Target
See all NFKBIA AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Specificity
- This antibody recognises IkB alpha, and identifies a single polypeptide band of 39 kDa in Western blots.
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Purification
- Affinity chromatography
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Immunogen
- Human IkB alpha expressed in E. coli
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anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (N-Term) antibody
NFKBIA Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 74-247) antibodyNFKBIA Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (pSer32), (pSer36) antibodyNFKBIA Reactivity: Human, Mouse, Rat WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 1-317) antibodyNFKBIA Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 150-291) antibodyNFKBIA Reactivity: Human IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 4D4C4 unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 3-112) antibodyNFKBIA Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 1-317) antibodyNFKBIA Reactivity: Human WB, ELISA, IF Host: Mouse Monoclonal 3H4 unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (AA 1-120) antibodyNFKBIA Reactivity: Human, Mouse, Rat, Cow, Chicken WB, ELISA, IF (p), IF (cc), FACS, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) antibodyKD Validated NFKBIA Reactivity: Human WB, IF (p), FACS Host: Rabbit Monoclonal 25GB4040 unconjugated Recombinant Antibody
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA) (Center) antibodyNFKBIA Reactivity: Human, Mouse WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated
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Application Notes
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ELISA: 1:2000. Western Blot: 1:200 - 1:1000. Immunoprecipitation. Gel Super Shift Assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS containing 0.09 % Sodium Azide and 0.5 % Bovine Serum Albumin
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- 4 °C/-20 °C
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Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch. -
Expiry Date
- 12 months
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- NFKBIA (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells Inhibitor, alpha (NFKBIA))
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Alternative Name
- NFKBIA / IKBA
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Background
- Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.Synonyms: I kappa B-alpha, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, Major histocompatibility complex enhancer-binding protein MAD3, NF-kappa-B inhibitor alpha, NFKBI
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Gene ID
- 4792
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UniProt
- P25963
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Pathways
- NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Maintenance of Protein Location, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, BCR Signaling
Target
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